Lhcb4 | CP29 (Lhcb4) homolog (Ostreococcus tauri)
AS09 512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri
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KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus tauri Q3B9U8
27 | 27 kDa
Bathycoccus prasinos, Micromonas sp.,
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1.0-4.5 µg of chlorophyll from Coccomyxa subellipsoidea cells were loaded to lanes. Samples were denatured with Laemmli buffer at 75°C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 115 seconds.
Courtesy MSc Paweł Rogowski, Warsaw University, Poland
Lhcb4 (CP29) is a member of the family of chlorophyll a/b-binding proteins, which is conserved in higher plants and green algae. Lhcb4 is associated with Photosystem II serving both as a light-harvesting antenna protein as well as playing a role in photoprotective dissipation of excitation energy. Alternative name: CP29-like light-harvesting chlorophyll a/b binding protein chlor (IC)
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