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Tag Antibody Set 

LOX | Lipoxygenase

AS06 128  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, G. max, L. longiflorum, L. luteus, O. europaea

LOX | Lipoxygenase in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS06 128)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

native lipoxygenase, type I-B, purified from Glycine max (Sigma, product number L7395) UniProt: P08170

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

54 (subunit), 108 (native enzyme)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Glycine max, Lilium longiflorum, Lupinus luteus, Olea europaea
Predicted reactivity

Glycine max, Lathyrus undulatus, Malus x domestica, Solanum tuberosum

Not reactive in

Chlamydomonas reinhardtii

Application examples

Application examples Application example

western blot using anti-LOX antibodies

Samples of Arabidopsis thaliana (2), Olea europaea (3), Lilium Longiflorum (4), Lupinus luteus (5) were ground in liquid nitrogen to a very fine powder using a mortar and pestle and resuspended in 1.5 ml of extraction buffer (4% SDS, 2% 2-mercaptoethanol, 2 mM PMSF, 100 mM Tris-HCl pH 8.5). The samples were incubated for 3 min at 80°C. Protein suspensions were clarified by centrifugation at 13,500 g for 10 min at room temperature and the resulting supernatants were used. Total proteins (25 µg per sample) were separated by SDS-PAGE on CriterionTMTGXTM Precast Gel (Bio-Rad, USA) using CriterionTM Cell apparatus (Bio-Rad). Proteins were electroblotted onto a PVDF membrane using Trans-Blot® TurboTM Transfer Pack (Bio-Rad) in a Trans-Blot® TurboTM Transfer System (Bio-Rad). The membrane was blocked for 1 h in solution containing 1 % (w/v) non-fat dry milk in TRIS-buffered saline (TBS) buffer, pH 7.4. The membrane was incubated in the primary antibody at a dilution of 1: 1000 in TBS buffer containing 1 % (w/v) non-fat dry milk over night at 4°C with agitation. A DyLight 488 conjugated anti-rabbit IgG (AS10 831, Agrisera), diluted 1:2000 in TBS buffer for 2 h, served as the secondary antibody. The signal was detected in a Pharos FX molecular imager (Bio-Rad). Line 1 contains LOX protein from Sigma. 

Courtesy of Dr. Agnieszka Zienkiewicz, CSIC, Spain

Additional information

Related products

Related products

AS06 128A | Anti-LOX | lipoxygenase, affinity purified, rabbit antibodies

AS07 258 | Anti-LOX-C | chloroplastic lipoxygenase, rabbit antibodies

AS08 340 | Anti-LOX2 | lipoxygenase 2 protein, rabbit antibodies

Plant protein extraction buffer

Background

Background

Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Synonymes: linoleate, oxygen oxidoreductase.

Product citations

Selected references Added when available. This antibody is a re-make of anti-LOX antibody sold until June 2013.

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