LOX-C | Lipoxygenase (chloroplastic)

Name: LOX-C | Lipoxygenase (chloroplastic)
Brand: 1
SKU: AS07 258
Price: 394 EUR
Availability: InStock
Rating: 5 (2 reviews)

Product no: AS07 258

AS07 258 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vitis vinifera

394 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	recombinant Arabidopsis thaliana protein, loop (aa 257-450) UniProt: P38418, TAIR: At3g45140
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 �l
Reconstitution:	For reconstitution add 100 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 50 000 (WB)
Expected \| apparent MW:	102 \| 97 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Vitis vinifera
Predicted reactivity:	Brassica napus, Musa acuminata subsp. malaccensis Species of your interest not listed? Contact us
Not reactive in:	Chlamydomonas reinhardtii

Additional Information
Additional information (application): Aweak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase
Background

Background:

Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2

Product Citations

Selected references:

Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941
Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R. (antibody used on LOX2 mutant plant)
Pilati et al. (2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
Reviews:
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Number of reviews: (2)

Stefania Pilati | 2013-04-04

0 | 0

This antibody recognizes with high specificity a Vitis vinifera chloroplastic lipoxygenase expressed in the berries along development (by western blotting). The lipoxygenase has been identified by nanoLC/MS.

Stefania Pilati | 2013-04-04

0 | 0

This antibody recognizes with high specificity a Vitis vinifera chloroplastic lipoxygenase expressed in the berries along development (by western blotting). The lipoxygenase has been identified by nanoLC/MS.