LOX-C | Lipoxygenase (chloroplastic)

AS07 258 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vitis vinifera

Product Information

Immunogen

recombinant Arabidopsis thaliana protein, loop (aa 257-450) UniProt: P38418, TAIR: At3g45140

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 50 000 (WB)

Expected | apparent MW

102 | 97 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Vitis vinifera

Predicted reactivity Brassica napus, Musa acuminata subsp. malaccensis

Not reactive in

Chlamydomonas reinhardtii

Application examples

Additional information

Aweak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase.

Related products

AS06 128 | Anti-LOX | lipoxygenase, rabbit antibodies

AS08 340 | Anti-LOX2 | lipoxygenase 2 protein. rabbit antibodies

Background

Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2

Product citations

Selected references Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941
Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R. (antibody used on LOX2 mutant plant)
Pilati et al. (2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87.