Llama anti-Mouse IgG (H&L), HRP Conjugated

AS15 2886 | Clonality: Polyclonal | Host: Llama | Reactivity: Mouse IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

Purified mouse IgG, whole molecule

Host Llama

Clonality Polyclonal

Purity Immunogen affinity purified llama IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications Immunoassays

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Reacts with: heavy (?) chains chains on mouse IgG light chains on all on all mouse immunoglobulins

Not reactive in

Based on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins

Application examples

Additional information

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.1% (v/v) Kathon CG

Based on IEP, this antibody reacts with: heavy (γ) chains chains on mouse IgG light chains on all on all mouse immunoglobulins.
Based on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins

Background

Llama anti-Mouse IgG (H&L), HRP Conjugated is a secondary antibody which binds to heavy (γ) chains chains on mouse IgG light chains on all mouse immunoglobulins

Product citations

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Confirmed reactivity:	Reacts with: heavy (?) chains chains on mouse IgG light chains on all on all mouse immunoglobulins
not reactive in:	Based on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins
background:	Llama anti-Mouse IgG (H&L), HRP Conjugated is a secondary antibody which binds to heavy (γ) chains chains on mouse IgG light chains on all mouse immunoglobulins
additional information:	10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.1% (v/v) Kathon CG Based on IEP, this antibody reacts with: heavy (γ) chains chains on mouse IgG light chains on all on all mouse immunoglobulins. Based on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Llama
immunogen:	Purified mouse IgG, whole molecule
Purity:	Immunogen affinity purified llama IgG.
Quantity:	1 mg
recommended dilution:	The optimal working dilution should be determined by the investigator
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	Immunoassays