LUC | Luciferase (firefly) (serum)

Product no: AS16 3691

AS16 3691 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Luciferase from Photinus pyralis (firefly)

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  • Data sheet

  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: Depends upon a MW of a protein which is LUC-tagged,
  • Reactivity
  • Confirmed reactivity: LUC
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-LUC antibodies

    total and cytosolic extract from Arabidopsis thaliana seedlings with detectable Luciferase expression at different zt time, three samples of a line with silenced luciferase expression ( no detection of Luciferin caused light emission ) and one wilde-type without CAB2::LUC expression. 100 µg of tissue was homogenized with extraction buffer on ice, spun at 4°C at 20 000g/15 min. and denatured at 100°C or 10 min.  Samples were separated on 12% SDS-PAGE and blotted over night to PVDF using semi-dry. Blots were blocked with milk-TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in for 1h at RT with agitation. The blot was washed as above and developed for min with ECL kit following manufacture recommendations.

    Courtesy of Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden
  • Background
  • Background: LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.
  • Product Citations
  • Selected references: Ormancey et al. (2023) Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nat Commun. 2023;14(1):254. Published 2023 Jan 17. doi:10.1038/s41467-023-35951-0
  • Protocols
  • Antibody protocols

     

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AS16 3691A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Luciferase from Photinus pyralis (firefly)

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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