LUC | Luciferase

PRODUCT INFORMATION IN PDF

product information Background LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production. Immunogen KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758 Host Rabbit Clonality Polyclonal Clone Purity Serum Format Lyophilized Quantity 50 µl Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western blot (WB) Related products Antibodies to GUS/GAL and other reporter systems Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected | apparent MW		Depends upon a MW of a protein which is LUC-tagged.
Confirmed reactivity		LUC
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		to be added when available, antibody released in Febraury 2017.

Application example

total and cytosolic extract from Arabidopsis thaliana seedlings with detectable Luciferase expression at different zt time, three samples of a line with silenced luciferase expression ( no detection of Luciferin caused light emission ) and one wilde-type without CAB2::LUC expression. 100 µg of tissue was homogenized with extraction buffer on ice, spun at 4°C at 20 000g/15 min. and denatured at 100°C or 10 min.  Samples were separated on 12% SDS-PAGE and blotted over night to PVDF using semi-dry. Blots were blocked with milk-TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in for 1h at RT with agitation. The blot was washed as above and developed for min with ECL kit following manufacture recommendations.

Courtesy of Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden

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