LUC | Luciferase (firefly) (serum)

Name: LUC | Luciferase (firefly) (serum)
SKU: AS16 3691
Price: 302 EUR
Availability: InStock

AS16 3691 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Luciferase from Photinus pyralis (firefly)

LUC | Luciferase (firefly) (serum) in the group Tag Antibodies / aadA1/BAR/BC2/BirA/CBP/GAL4/GUS/LUC at Agrisera AB (Antibodies for research) (AS16 3691)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW Depends upon a MW of a protein which is LUC-tagged,

Reactivity

Confirmed reactivity LUC

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

total and cytosolic extract from Arabidopsis thaliana seedlings with detectable Luciferase expression at different zt time, three samples of a line with silenced luciferase expression ( no detection of Luciferin caused light emission ) and one wilde-type without CAB2::LUC expression. 100 µg of tissue was homogenized with extraction buffer on ice, spun at 4°C at 20 000g/15 min. and denatured at 100°C or 10 min. Samples were separated on 12% SDS-PAGE and blotted over night to PVDF using semi-dry. Blots were blocked with milk-TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in for 1h at RT with agitation. The blot was washed as above and developed for min with ECL kit following manufacture recommendations.

Courtesy of Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden

Additional information

Background

Background LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.

Product citations

Selected references Ormancey et al. (2023) Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nat Commun. 2023;14(1):254. Published 2023 Jan 17. doi:10.1038/s41467-023-35951-0

immunogen:	KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	Depends upon a MW of a protein which is LUC-tagged,
Confirmed reactivity:	LUC
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example total and cytosolic extract from Arabidopsis thaliana seedlings with detectable Luciferase expression at different zt time, three samples of a line with silenced luciferase expression ( no detection of Luciferin caused light emission ) and one wilde-type without CAB2::LUC expression. 100 µg of tissue was homogenized with extraction buffer on ice, spun at 4°C at 20 000g/15 min. and denatured at 100°C or 10 min. Samples were separated on 12% SDS-PAGE and blotted over night to PVDF using semi-dry. Blots were blocked with milk-TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:5000 in for 1h at RT with agitation. The blot was washed as above and developed for min with ECL kit following manufacture recommendations. Courtesy of Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden
background:	LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.
All references:	Ormancey et al. (2023) Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nat Commun. 2023;14(1):254. Published 2023 Jan 17. doi:10.1038/s41467-023-35951-0

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