Lysine-tRNA ligase

Name: Lysine-tRNA ligase
SKU: AS15 2899
Price: 302 EUR
Availability: InStock

AS15 2899 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Lysine-tRNA ligase in the group Antibodies Plant/Algal / Plant Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS15 2899)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 500 (WB)

Expected | apparent MW

41 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Application examples

Application example

20 μg of protein from Arabidopsis thylakoids or chloroplasts were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 Agrisera ) diluted to 1: 8 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 minute.

Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.

Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands

Additional information

This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system

Background

Background Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm.

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 500 (WB)
calculated \| apparent molecular mass [kDa]:	41 kDa
Confirmed reactivity:	Arabidopsis thaliana
Picture (footer):	Application example 20 μg of protein from Arabidopsis thylakoids or chloroplasts were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 Agrisera ) diluted to 1: 8 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 minute. Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above. Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands
additional information (application):	This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system
background:	Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm.

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