AS15 2899 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
|Data sheet||Product citations||Protocols||Add review|
Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.
Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands
Related products: Lysine-tRNA ligase
This product can b...
This product can be purchased in 3 different volum...