Lysine-tRNA ligase
AS15 2899 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
41 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Application examples
Application examples
Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.
Application example
Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.
Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands
Additional information
This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system
Background
Background
Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm.
Product citations
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