MPK6 | Mitogen-activated protein kinase 6
AS12 2633 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MPK6 protein, UniProt: Q39026, TAIR: At2g43790
45 kDa
Reactivity
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Application examples
Application example
0.2g of 21 days old Arabidopsis thaliana leaf tssue C=Col, 6= mpk6 (T-DNA mutant), 3= mpk3 (T-DNA mutant) was homogenized with 250 μ l Buffer A( 50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl , complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material. Material was extracted with 250 μl of Buffer A (50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl, complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material. Supernatant (120 μ L)+ 4XSDS sample buffer 40 μ L= 200 μ L samples ↓ 95 °C 5min 10 μ L were separated on 10 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with skim milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in TBS-T at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescence according to the manufacturer's instructions.
Courtesy of Chika Tateda, University of Chicago, USA
Additional information
Background
Product citations
Wang and Auwerx (2017). Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress. Mol Cell. 2017 Nov 2;68(3):540-551.e5. doi: 10.1016/j.molcel.2017.10.006.
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