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MPK6 | Mitogen-activated protein kinase 6

AS12 2633 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

MPK6 | Mitogen-activated protein kinase 6 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2633)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MPK6 protein, UniProt: Q39026, TAIR: At2g43790

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Gossypium mexicanum
Species of your interest not listed? Contact us
Not reactive in Solanum tuberosum

Application examples

Application examples

Application example

western blot using Agrisera anti-MPK6 antibodies


0.2g of 21 days old Arabidopsis thaliana leaf tssue C=Col, 6= mpk6 (T-DNA mutant), 3= mpk3 (T-DNA mutant) was homogenized with 250 μ l Buffer A( 50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl , complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material.   Material was extracted with  250 μl of Buffer A (50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl, complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material. Supernatant (120 μ L)+ 4XSDS sample buffer 40 μ L= 200 μ L samples ↓ 95 °C 5min 10 μ L were separated on 10 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with skim milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in TBS-T at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:75 000 in  for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescence according to the manufacturer's instructions.

Courtesy of Chika Tateda, University of Chicago, USA

Additional information

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Plant protein extraction buffer

Secondary antibodies

Background

Background MPK6 (Mitogen-activated protein kinase 6) is involved in oxidative stress-mediated signaling cascade, in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. Activated in response to touch, wounding, low temperature, low humidity, salt stress, hydrogen peroxide, ozone, ACC (an ethylene precursor), jasmonic acid (JA), mastoparan and UVC. Activated in response to elicitors: oligogalacturonides, hexameric chitin fragments, fungal xylanase, and the bacterial flagellin and harpin. Synonymes: MAP kinase 6, AtMPK6.

Product citations

Selected references Butler et al. (2019). Soybean resistance locus Rhg1 confers resistance to multiple cyst nematodes in diverse plant species. Phytopathology. 2019 Aug 12. doi: 10.1094/PHYTO-07-19-0225-R.
Wang and Auwerx (2017). Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress. Mol Cell. 2017 Nov 2;68(3):540-551.e5. doi: 10.1016/j.molcel.2017.10.006.

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