RE | N-terminal arginylation

AS15 3050 | Clonality: Polyclonal | Host: Rabbit | Reactivity: RE | N-terminal arginylation

Product Information

Immunogen

KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 2x50 µg

Reconstitution For reconstitution add 50 µl of sterile water to each tube

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Dot blot (Dot)

Recommended dilution

1 : 1000 (Dot)

Reactivity

Application examples

Application examples Application information

Membrane: PVDF, Hybond 0.45 μm (GE Healthcare)
0.5 µg synthetic peptide per spot
Membrane was blocked overnight in TBST, 4% Blocking Agent (GE Healthcare)
Primary antibodies: 1:10 000 in TBST 2% Blocking Agent
Secondary antibody (AS09 602): 1:25 000 in TBST 2% Blocking Agent
Detection: chemiluminescent detection reagent

Courtesy of Dr. Sebastian Hoernstein, Faculty of Biology, University of Freiburg; Germany

Additional information

Additional information Antibody is purified using substractive purification method

MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins

Background

Background Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.

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Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	2x50 µg
recommended dilution:	1 : 1000 (Dot)
Reconstitution:	For reconstitution add 50 µl of sterile water to each tube
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Dot blot (Dot)
Picture (footer):	Application information Membrane: PVDF, Hybond 0.45 μm (GE Healthcare) 0.5 µg synthetic peptide per spot Membrane was blocked overnight in TBST, 4% Blocking Agent (GE Healthcare) Primary antibodies: 1:10 000 in TBST 2% Blocking Agent Secondary antibody (AS09 602): 1:25 000 in TBST 2% Blocking Agent Detection: chemiluminescent detection reagent Courtesy of Dr. Sebastian Hoernstein, Faculty of Biology, University of Freiburg; Germany
additional information:	Antibody is purified using substractive purification method
additional information (application):	MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins
background:	Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.