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RE | N-terminal arginylation

AS15 3050 | Clonality: Polyclonal | Host: Rabbit | Reactivity: RE | N-terminal arginylation

RE | N-terminal arginylation in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS15 3050)

DATA SHEET IN PDF

Qty: 
455
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 2x50 µg
Reconstitution For reconstitution add 50 µl of sterile water to each tube
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot)
Recommended dilution

1 :  1000 (Dot)

Reactivity

Application examples

Application examples Application information

Dot blot using anti-arginylation antibodies

  • Membrane: PVDF, Hybond 0.45 μm (GE Healthcare)
  • 0.5 µg synthetic peptide per spot
  • Membrane was blocked overnight in TBST, 4% Blocking Agent (GE Healthcare)
  • Primary antibodies: 1:10 000 in TBST 2% Blocking Agent
  • Secondary antibody (AS09 602): 1:25 000 in TBST 2% Blocking Agent
  • Detection: chemiluminescent detection reagent


Courtesy of Dr. Sebastian Hoernstein, Faculty of Biology, University of Freiburg; Germany

Additional information

Additional information Antibody is purified using substractive purification method.
MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins.

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Background

Background Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.

Product citations

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