NAB1 | nucleic acid binding protein 1, Chlamydomonas
AS08 333 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of Chlamydomonas cytoplasm
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His-tagged recombinant NAB1 from Chlamydomonas reinhardtii UniProt:Q8GV23
26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)
Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160 000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.
NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.
In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).
Desai et al. (2014). Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes. Cytoskeleton (Hoboken). 2014 Dec 31. doi: 10.1002/cm.21206.
Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421