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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

NAB1 | nucleic acid binding protein 1, Chlamydomonas

AS08 333 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of Chlamydomonas cytoplasm

NAB1 | nucleic acid binding protein 1, Chlamydomonas in the group Plant/Algal Antibodies / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS08 333)

PRODUCT INFORMATION IN PDF

Qty: 
272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

Info: Add review
product information
Background

In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).

Immunogen

His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Related products

Collection of antibodies to Chlamydomonas proteins

Additional information

NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.

application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlamydomonas reinhardtii
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Craft et al. (2015). Tubulin transport by IFT is upregulated during ciliary growth by a cilium-autonomous mechanism. J Cell Biol. 2015 Jan 19;208(2):223-37. doi: 10.1083/jcb.201409036. Epub 2015 Jan 12.
Desai et al. (2014). Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes. Cytoskeleton (Hoboken). 2014 Dec 31. doi: 10.1002/cm.21206.
Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421

application example

Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.


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