NAD6 | NADH-ubiquinone oxidoreductase chain 6
AS15 2926 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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23.4 kDa (Arabidopsis thaliana), 25 kDa (Zea mays)
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12 µg of Arabidopsis thaliana leaf mitochondrial protein (Mini-protean 3, BioRad) denatured with 2x Laemmli solubilisation buffer at 65ºC for 5-10 min were separated on 16 % SDS-PAGE and blotted 45 min to PVDF using semi-dry transfer. Blots were blocked with commercial blocker from Roche at 1:10 dilution for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera AS09 602) diluted to 1: 15 000 in for 1h at RT with agitation. The blot was washed as above and developed for min withchemiluminescent detection reagent. Exposure time was 1 min for the blots probed with the purified antibody. Green arrow is pointing out at contamination band present in a mutant.
Courtesy of Dr. Catherine Colas des Francs-Small, UWA, Australia
Colas des Francs-Small et al. (2018). Targeted cleavage of nad6 mRNA induced by a modified pentatricopeptide repeat protein in plant mitochondria. Commun Biol. 2018 Oct 11;1:166. doi: 10.1038/s42003-018-0166-8.
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