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NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic)

AS16 4064 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS16 4064)

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Product Information

Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhB protein sequence, UniProt: P0CC32, P0CC33, TAIR: AtCg00890, ATCG01250
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Tested applications Western blot (WB)
Recommended dilution

1:1000 (WB)

Expected | apparent MW

35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Anastatica hierochuntica, Arabis stelleri, Barbarea verna, Braya humilis, Bunias orientalis, Cakile arabica, Camelina sativa, Cannabis sativa, Capsella bursa-pastoris, Capsella rubella, Cardamine impatiens, Chorispora tenella, Cleomella serrulata, Cochlearia pyrenaica, Crucihimalaya wallichii, Dontostemon micranthus, Euclidium syriacum, Eutrema yunnanense, Farsetia stylosa, Fragaria ananassa, Hesperis matronalis, Ionopsidium acaule, Lepidium virginicum, Lobularia maritima, Megadenia pygmaea, Matthiola incana, Nasturtium officinale, Neotorularia korolkowii, Noccaea caerulescens, Olimarabidopsis pumila, Orychophragmus taibaiensis, Oryza sativa, Pachycladon enysii, Phaseolus vulgaris, Physaria ludoviciana, Pugionium cornutum, Schrenkiella parvula, Tarenaya hassleriana, Thlaspi arvense
Species of your interest not listed? Contact us
Not reactive in cyanobacteria

Application examples

Application examples Application example

Western blot using anti-NdhB antibodies

5µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 mM Tris pH7.5, 10% glycerol, 1 mM EDTA, 1mM EGTA, 2mM pMSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 mM DTT) and denatured in 1XPSB (33 mM Tris pH 6.8, 50 mM 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70C for 5 min, separated on 4-20% Tris-Glycine SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked with 10% milk in 1XTBSt for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation in 10% milk in 1XTBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10,000 in 10% milk in 1XTBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent.

Courtesy Rosalind Carrier, University of Oregon, USA

Additional information

Related products

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AS13 2711 | Anti-NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic), rabbit antibodies
AS16 4065 | Anti-NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic), rabbit antibodies
AS16 4066 | Anti-NdhS | NAD(P)H-quinone oxidoreductase subunit S (chloroplastic), rabbit antibodies 

antibodies to proteins involved in electron transfer in chloroplasts

Secondary antibodies

Background

Background

NAD(P)H-quinone oxidoreductase subunit 2 is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names: NAD(P)H dehydrogenase, subunit 2, NADH-plastoquinone oxidoreductase subunit 2.

Product citations

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