NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)
AS16 4065 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhH protein sequence, UniProt:P56753-1, TAIR: ATCG01110
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1:5000 (WB)
Expected | apparent MW
45 | 49 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Zea mays
Predicted reactivity
Cannabis sativa, Hordeum vulgare, Microcystis aeruginosa PCC 7806, Physcomitrella patens, Synechocystis sp.
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Pisum sativum, Phaseolus vulgaris
Application examples
Application examples
Application example
Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8M urea, 100 mM Tris-HCL pH 7.5, 1 mM EDTA, 2% SDS. 10 ug of TCA precipites were separated in a BioRad TGX 4-20% precast gels and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8 % milk in TTBS for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.
Courtesy Msc. Lauri Nikkanen, University of Turku, Finland
Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8M urea, 100 mM Tris-HCL pH 7.5, 1 mM EDTA, 2% SDS. 10 ug of TCA precipites were separated in a BioRad TGX 4-20% precast gels and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8 % milk in TTBS for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.
Courtesy Msc. Lauri Nikkanen, University of Turku, Finland
Additional information
This product can be sold containing proclin if requested
Background
Background
NAD(P)H dehydrogenase subunit H (EC=1.6.5) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names: NAD(P)H dehydrogenase subunit H; NDH7
NADH-plastoquinone oxidoreductase 49 kDa subunit, NADH-plastoquinone oxidoreductase subunit H.
Product citations
Selected references
Seiml-Buchinger et al. (2022) Ascorbate peroxidase postcold regulation of chloroplast NADPH dehydrogenase activity controls cold memory. Plant Physiol. 2022;190(3):1997-2016. doi:10.1093/plphys/kiac355
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.
Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv, doi: https://doi.org/10.1101/261560.
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.
Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv, doi: https://doi.org/10.1101/261560.
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