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NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)

AS16 4065 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic) in the group Antibodies Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS16 4065)
NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)



DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhH protein sequence, UniProt:P56753-1, TAIR: ATCG01110

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution

1:5000 (WB)

Expected | apparent MW

45 | 49 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Cannabis sativa, Hordeum vulgare, Microcystis aeruginosa PCC 7806, Physcomitrella patens, Synechocystis sp.

Species of your interest not listed? Contact us
Not reactive in Pisum sativum, Phaseolus vulgaris

Application examples

Application examples Application example


Western blot using anti-NdhH antibodies on Arabidopsis thaliana

Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8M urea, 100 mM Tris-HCL pH 7.5, 1 mM EDTA, 2% SDS. 10 ug of TCA precipites were separated in a BioRad TGX 4-20% precast gels and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8 % milk in TTBS for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy Msc. Lauri Nikkanen, University of Turku, Finland

Additional information

This product can be sold containing proclin if requested

Related products

Background

Background

NAD(P)H dehydrogenase subunit H (EC=1.6.5) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur  centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names:  NAD(P)H dehydrogenase subunit H; NDH7
NADH-plastoquinone oxidoreductase 49 kDa subunit, NADH-plastoquinone oxidoreductase subunit H.

Product citations

Selected references Seiml-Buchinger et al. (2022) Ascorbate peroxidase postcold regulation of chloroplast NADPH dehydrogenase activity controls cold memory. Plant Physiol. 2022;190(3):1997-2016. doi:10.1093/plphys/kiac355
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.
Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv, doi: https://doi.org/10.1101/261560.
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhH protein sequence, UniProt:P56753-1, TAIR: ATCG01110

Reconstitution: For reconstitution add 50 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 µg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution:

1:5000 (WB)

calculated | apparent molecular mass [kDa]:

45 | 49 kDa

Confirmed reactivity: Arabidopsis thaliana, Zea mays
predicted reactivity: Cannabis sativa, Hordeum vulgare, Microcystis aeruginosa PCC 7806, Physcomitrella patens, Synechocystis sp.

Species of your interest not listed? Contact us
not reactive in: Pisum sativum, Phaseolus vulgaris
Picture (footer): Application example


Western blot using anti-NdhH antibodies on Arabidopsis thaliana

Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8M urea, 100 mM Tris-HCL pH 7.5, 1 mM EDTA, 2% SDS. 10 ug of TCA precipites were separated in a BioRad TGX 4-20% precast gels and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8 % milk in TTBS for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy Msc. Lauri Nikkanen, University of Turku, Finland
additional information (application): This product can be sold containing proclin if requested
background:

NAD(P)H dehydrogenase subunit H (EC=1.6.5) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur  centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names:  NAD(P)H dehydrogenase subunit H; NDH7
NADH-plastoquinone oxidoreductase 49 kDa subunit, NADH-plastoquinone oxidoreductase subunit H.

All references: Seiml-Buchinger et al. (2022) Ascorbate peroxidase postcold regulation of chloroplast NADPH dehydrogenase activity controls cold memory. Plant Physiol. 2022;190(3):1997-2016. doi:10.1093/plphys/kiac355
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.
Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv, doi: https://doi.org/10.1101/261560.

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