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NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)

AS16 4065 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS16 4065)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhH protein sequence, UniProt:P56753-1, TAIR: ATCG01110

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution

1:5000 (WB)

Expected | apparent MW

45 | 49 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Cannabis sativa, Hordeum vulgare, Microcystis aeruginosa PCC 7806, Synechocystis sp.

Species of your interest not listed? Contact us
Not reactive in Pisum sativum, Phaseolus vulgaris

Application examples

Application examples Application example


Western blot using anti-NdhH antibodies on Arabidopsis thaliana

Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8M urea, 100 mM Tris-HCL pH 7.5, 1 mM EDTA, 2% SDS. 10 ug of TCA precipites were separated in a BioRad TGX 4-20% precast gels and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8 % milk in TTBS for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy Msc. Lauri Nikkanen, University of Turku, Finland

Additional information

This product can be sold containing proclin if requested.

Related products

Related products AS16 4064 | Anti-NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic), rabbit antibodies
AS13 2711 | Anti-NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic), rabbit antibodies
AS16 4066 | Anti-NdhS | NAD(P)H-quinone oxidoreductase subunit S (chloroplastic), rabbit antibodies 

antibody collection to proteins involved in photosynthetic electron transfer

Secondary antibodies

Background

Background

NAD(P)H dehydrogenase subunit H (EC=1.6.5) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur  centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names:  NAD(P)H dehydrogenase subunit H; NDH7
NADH-plastoquinone oxidoreductase 49 kDa subunit, NADH-plastoquinone oxidoreductase subunit H.

Product citations

Selected references Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv, doi: https://doi.org/10.1101/261560.

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