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Na+/H+ antiporter, sodium/hydrogen exchanger

AS09 484 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Na+/H+ antiporter, sodium/hydrogen exchanger  in the group Antibodies for Plant/Algal / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 484)

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product information
Background

Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membraes. Localized in tonoplast, possibily also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt:  Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Protocol for vacuolar membrane isolation can be found here.

application information
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

59.5 | 45 kDa

Confirmed reactivity Arabidopsis thaliana, Kandelia obovata, Solanum lycopersicum
Predicted reactivity Gossypium hirsutum, Oryza sativa, Populus euphratica, Ricinus communis, Zea mays, Vitis vinifera
Not reactive in

Mangrove plants, Nicotiana benthamiana, sp. Avicennia,

Additional information
Selected references Kumari et al. (2017), Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Front Plant Sci. 2017 Jan 6;7:2027. doi: 10.3389/fpls.2016.02027.
Chen et al. (2013). Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity. 8(8): e71543. doi:10.1371/journal.pone.0071543.

application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana (left panel)and1,5 and 10 µg of crude membrane fraction/lane Raphanus sativus L.(right panel)were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-NHX antibodies (AS09 484, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

 

western blot detection using NHX antibodies

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