Nitrotyrosine (mouse monoclonal) (clone EM-30)

AS21 4622 | Clonality: Monoclonal | Host: Mouse | Reactivity: nitrotyrosine

Nitrotyrosine (mouse monoclonal) (clone EM-30)

Product Information

Immunogen NO_2-Tyr-CH2-Thyroglobulin

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunoglobulin purified on Protein A.

Format Liquid at 1 mg/ml.

Quantity 0,1 mg

Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)

Recommended dilution (IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(WB): positive control: NO2-BSA, NO2-HSA.

Reactivity

Confirmed reactivity Nitrotyrosine

Application examples

Application examples IHC using mouse monoclonal anti-nitrotyrosine antibodies

Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).

Additional information

Additional information This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues

Background

Background Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

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additional information:	This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues
Clonality:	Monoclonal
Format:	Liquid at 1 mg/ml.
Host:	Mouse
immunogen:	NO_2-Tyr-CH2-Thyroglobulin
Purity:	Immunoglobulin purified on Protein A.
Quantity:	0,1 mg
recommended dilution:	(IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (WB): positive control: NO2-BSA, NO2-HSA.
storage:	Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class:	IgG1
tested applications:	Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
Picture (footer):	Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).
Confirmed reactivity:	Nitrotyrosine
background:	Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.