Nitrotyrosine (mouse monoclonal) (clone EM-30)
AS21 4622 | Clonality: Monoclonal | Host: Mouse | Reactivity: nitrotyrosine

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Product Information
Immunogen
NO2-Tyr-CH2-Thyroglobulin
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Immunoglobulin purified on Protein A.
Format
Liquid at 1 mg/ml.
Quantity
0,1 mg
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
Recommended dilution
(IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(WB): positive control: NO2-BSA, NO2-HSA.
(IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(WB): positive control: NO2-BSA, NO2-HSA.
Reactivity
Confirmed reactivity
Nitrotyrosine
Application examples
Application examples

Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).
Additional information
Additional information
This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues
Background
Background
Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
Product citations
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