Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)

Name: Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)
SKU: AS21 4622
Price: 315 EUR
Availability: InStock

Product no: AS21 4622

AS21 4622 | Clonality: Monoclonal | Host: Mouse | Reactivity: nitrotyrosine

315 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG1
Immunogen:	NO_2-Tyr-CH2-Thyroglobulin
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin purified on Protein A.
Format:	Liquid at 1 mg/ml.
Quantity:	0.1 mg
Storage:	Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
Recommended dilution:	(IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (WB): positive control: NO2-BSA, NO2-HSA.

Reactivity
Confirmed reactivity: Nitrotyrosine
Application Examples
Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).
Additional Information
Additional information: This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues
Background

Background:

Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

Protocols
Agrisera Western Blot protocol and video tutorials

Antibody Protocols
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