Nitrotyrosine (mouse monoclonal) (clone EM-30)

Name: Nitrotyrosine (mouse monoclonal) (clone EM-30)
SKU: AS21 4622
Price: 290 EUR
Availability: InStock

AS21 4622 | Clonality: Monoclonal | Host: Mouse | Reactivity: nitrotyrosine

Nitrotyrosine (mouse monoclonal) (clone EM-30) in the group Antibodies Human Cell Biology / Human Oxidative Stress at Agrisera AB (Antibodies for research) (AS21 4622)

Nitrotyrosine (mouse monoclonal) (clone EM-30)

Product Information

Immunogen NO_2-Tyr-CH2-Thyroglobulin

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunoglobulin purified on Protein A.

Format Liquid at 1 mg/ml.

Quantity 0,1 mg

Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)

Recommended dilution (IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
(WB): positive control: NO2-BSA, NO2-HSA.

Reactivity

Confirmed reactivity Nitrotyrosine

Application examples

Application examples IHC using mouse monoclonal anti-nitrotyrosine antibodies

Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).

Additional information

Additional information This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues

Background

Background Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

Product citations

Related products: Nitrotyrosine (mouse monoclonal) (clone EM-30)

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only...

From 26 €

Info

Add to cart

Goat anti-Mouse IgG (H&L), DyLight® 550 conjugated

AS12 1938 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG ...

165 €

Goat anti-Mouse IgG (H&L), DyLight® 633 conjugated

AS12 2184 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG

165 €

Rabbit anti-Mouse IgG (H&L), HRP conjugated

AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L...

206 €

immunogen:	NO_2-Tyr-CH2-Thyroglobulin
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin purified on Protein A.
Format:	Liquid at 1 mg/ml.
Quantity:	0,1 mg
storage:	Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
recommended dilution:	(IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (WB): positive control: NO2-BSA, NO2-HSA.
Confirmed reactivity:	Nitrotyrosine , Nitrotyrosine
Picture (footer):	Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).
additional information:	This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues
background:	Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.