Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)
AS21 4577 | Clonality: Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Detection antibody)

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Product Information
Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
Format
Liquid
Quantity
100 ĩl
Storage
Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
Expected | apparent MW
48 | 55 kDa
Reactivity
Confirmed reactivity
Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
Application examples
Application examples

Recombinant SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.


Recombinant SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36 to 23.97 ng/ml.
Additional information
Background
Background
Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).
Alternative names: NC, Protein N, Nucleocapsid protein
Alternative names: NC, Protein N, Nucleocapsid protein
Product citations
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