IKP

Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)

Product no: AS21 4577

AS21 4577   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Detection antibody)

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  • Product Info
  • Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9

    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C or -80C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
    Expected | apparent MW: 48 | 55 kDa
  • Reactivity
  • Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
  • Application Examples
  • Western blot using anti Nucleoprotein N monoclonal antibodies


    Recombinant  SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

    Binding activity of anti-nucleoprotein N monoclonal antibodies

    ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
    Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36  to 23.97 ng/ml.

  • Additional Information
  • Additional information: This is a detection antibody, which can be combined with Capture antibody:
    AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies and Positive control: 
    AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)
  • Background
  • Background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

    Alternative names:
    NC, Protein N, Nucleocapsid protein
  • Protocols
  • Antibody protocols
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(Capture antibody)

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