Name:
Phone:
E-mail:
Address:


 


Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)

AS21 4577   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Detection antibody)

Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody) in the group Antibodies for Human/Animal  / Coronavirus Research at Agrisera AB (Antibodies for research) (AS21 4577)

DATA SHEET IN PDF

Qty: 
255
Buy 2 items of this product for 178.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300
Format Liquid
Quantity 100 µl
Storage Store at -20°C or -80°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
Expected | apparent MW 48 | 55 kDa

Reactivity

Confirmed reactivity Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV

Application examples

Application examples Western blot using anti Nucleoprotein N monoclonal antibodies


Recombinant  SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

Binding activity of anti-nucleoprotein N monoclonal antibodies

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36  to 23.97 ng/ml.

Additional information

Additional information This is a detection antibody, which can be combined with Capture antibody:
AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies and Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Related products

Related products Capture antibody:
AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies

Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Products for coronovirus research

Background

Background Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

Alternative names:
NC, Protein N, Nucleocapsid protein

Product citations

Related products: Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)

AS15 TMB-HRP | TMB based, especially formulated with extreme sensitivity, HRP substrate for microw...
From 10 €
AS11 1772 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)


161 €
AS20 4388 | Recombinant protein (positive control) for Nucleoprotein (N) of Novel Coronavirus SARS...
From 194 €
AS21 4576   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N)...
255 €
Buy 2 items of this product for 178.00 €/items