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Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)

AS21 4577   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Detection antibody)

Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody) in the group Antibodies Human Cell Biology / Coronavirus Research at Agrisera AB (Antibodies for research) (AS21 4577)



DATA SHEET IN PDF

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Product Information

Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
Format Liquid
Quantity 100 ĩl
Storage Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
Expected | apparent MW 48 | 55 kDa

Reactivity

Confirmed reactivity Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV

Application examples

Application examples Western blot using anti Nucleoprotein N monoclonal antibodies


Recombinant  SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

Binding activity of anti-nucleoprotein N monoclonal antibodies

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36  to 23.97 ng/ml.

Additional information

Additional information This is a detection antibody, which can be combined with Capture antibody:
AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies and Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Related products

Background

Background Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

Alternative names: NC, Protein N, Nucleocapsid protein

Product citations

immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Sub class: IgG1
Host: Mouse
Clonality: Monoclonal
Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
Format: Liquid
Quantity: 100 ĩl
storage: Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Western blot (WB)
recommended dilution: 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
calculated | apparent molecular mass [kDa]: 48 | 55 kDa
Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
Picture (footer): Western blot using anti Nucleoprotein N monoclonal antibodies


Recombinant  SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

Binding activity of anti-nucleoprotein N monoclonal antibodies

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36  to 23.97 ng/ml.
additional information: This is a detection antibody, which can be combined with Capture antibody:
AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies and Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)
background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

Alternative names: NC, Protein N, Nucleocapsid protein

Related products: Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)

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