Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)
AS21 4577 | Clonality: Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
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Recombinant SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.
ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36 to 23.97 ng/ml.
Alternative names: NC, Protein N, Nucleocapsid protein