Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (detection antibody)
Product no: AS21 4577
AS21 4577 | Clonality: Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Detection antibody)
393
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300. Format: Liquid Quantity: 100 µl Storage: Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB) Expected | apparent MW: 48 | 55 kDa - Reactivity
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Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
- Application Examples
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Recombinant SARS-CoV-2 nucleocapsid protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size: 0.45 µm). Following the blocking in 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.
ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 15.36 to 23.97 ng/ml. - Additional Information
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Additional information: This is a detection antibody, which can be combined with Capture antibody:
AS21 4576 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies and Positive control:
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N) - Background
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Background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).
Alternative names: NC, Protein N, Nucleocapsid protein
- Protocols
- Antibody protocols
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