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Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)

AS21 4576   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Capture antibody)

Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody) in the group Antibodies for Human/Animal  / Coronavirus Research at Agrisera AB (Antibodies for research) (AS21 4576)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300
Format Liquid
Quantity 100 µl
Storage Store at -20°C or -80°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
Expected | apparent MW 48 | 55 kDa

Reactivity

Confirmed reactivity Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV

Application examples

Application examples Western blot using anti Nucleoprotein N antibodies


Recombinant  SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm). Following the blocking with 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

Functional ELISA showing binding activity of anti-SARS-CoV protein N antibodies

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 27.78 to 41.57 ng/ml.

Additional information

Additional information This product is a capture antibody, which can be combined with Detection antibody: 
AS21 4577 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodiesand Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Related products

Related products Detection antibody: 
AS21 4577 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodies

Positive control: 
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Products for coronovirus research

Background

Background Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

Alternative names:
NC, Protein N, Nucleocapsid protein

Product citations

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