Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)

AS21 4576 | Clonality: Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Capture antibody)

Product Information

Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.

Format Liquid

Quantity 100 µl

Storage Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)

Expected | apparent MW 48 | 55 kDa

Reactivity

Confirmed reactivity Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV

Application examples

Application examples Western blot using anti Nucleoprotein N antibodies

Recombinant SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm). Following the blocking with 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

Functional ELISA showing binding activity of anti-SARS-CoV protein N antibodies

Functional ELISA showing binding activity of anti-SARS-CoV protein N antibodies

ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 27.78 to 41.57 ng/ml.

Additional information

Additional information This product is a capture antibody, which can be combined with Detection antibody:
AS21 4577 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodiesand Positive control:
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)

Background

Background Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

Alternative names: NC, Protein N, Nucleocapsid protein

Product citations

calculated \| apparent molecular mass [kDa]:	48 \| 55 kDa
Clonality:	Monoclonal
Format:	Liquid
Host:	Mouse
immunogen:	Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Purity:	Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
Quantity:	100 µl
recommended dilution:	1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
storage:	Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class:	IgG1
tested applications:	ELISA (ELISA), Western blot (WB)
Picture (footer):	Recombinant SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm). Following the blocking with 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence. ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA. Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 27.78 to 41.57 ng/ml.
additional information:	This product is a capture antibody, which can be combined with Detection antibody: AS21 4577 \| Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodiesand Positive control: AS20 4388 \| Human Novel Coronavirus Nucleoprotein(N)
Confirmed reactivity:	Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
background:	Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP). Alternative names: NC, Protein N, Nucleocapsid protein

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