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Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)
Product no: AS21 4576
AS21 4576 | Clonality: Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Capture antibody)
393
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Sub class: IgG1 Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
Host: Mouse Clonality: Monoclonal Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300. Format: Liquid Quantity: 100 µl Storage: Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB) Expected | apparent MW: 48 | 55 kDa - Reactivity
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Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
- Application Examples
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Recombinant SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm). Following the blocking with 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.
ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 27.78 to 41.57 ng/ml. - Additional Information
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Additional information: This product is a capture antibody, which can be combined with Detection antibody:
AS21 4577 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodiesand Positive control:
AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)
- Background
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Background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).
Alternative names: NC, Protein N, Nucleocapsid protein
- Protocols
- Antibody protocols
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