PAB | protein in chloroplast atpase biogenesis

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AS15 2833 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays


28 st
Item No:
AS15 2833

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product information
Background PAB (protein in chloroplast atpase biogenesis) acts as an assembly chaperone that functions downstream of chaperonin 60 in the assembly of chloroplast ATP synthase coupling factor 1.

Recombinant PAB from Zea mays, GRMZM2G110258

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

antibodies to ATP synthase

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

35 kDa (without transit peptide)

Confirmed reactivity Arabidiopsis thaliana, Zea mays
Predicted reactivity Brachypodium distachyon, Gossypium raimondii, Hordeum vulgare, Oryza sp., Saccharum hybrid cultivar R570, Setaria italica, Sorghum bicolor, Theobroma cacao, Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in June 2015

application information

western blot using anti-PAB antibodies on maize tissue

western blot using anti-PAB antibodies on Arabidopsis thaliana tissue

Lanes contain ~5 µg of total protein from Zea mays seedling leaves (upper image), Arabidopsis thaliana seedling leaves (lower image), or isolated chloroplast stroma. Leaf extract from homozygous pab mutants (hypomorphic alleles), and purified recombinant antigen were analyzed as controls. Proteins were separated on 12% SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked for 45 min at room temperature (RT) in TBST +4% dry milk with agitation. The blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed twice in TBST, then washed three times for 10 min in TBST at RT with agitation. The blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in TBST +1% dry milk for 1h at RT with agitation. The blot was washed as above and incubated for ~2 minutes in ECL reagents prior to imaging with a LiCor digital imager. Exposure time was ~30 seconds.

Courtesy of Dr. Alison Barkan, The University of Oregon, USA

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