PAB | protein in chloroplast atpase biogenesis

Product no: AS15 2833

AS15 2833 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

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  • Product Info
  • Immunogen:

    Recombinant PAB from Zea mays, GRMZM2G110258

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    35 kDa (without transit peptide)

  • Reactivity
  • Confirmed reactivity: Arabidiopsis thaliana, Zea mays
    Predicted reactivity: Brachypodium distachyon, Gossypium raimondii, Hordeum vulgare, Oryza sp., Saccharum hybrid cultivar R570, Setaria italica, Sorghum bicolor, Theobroma cacao, Triticum aestivum
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application information

    western blot using anti-PAB antibodies on maize tissue


    western blot using anti-PAB antibodies on Arabidopsis thaliana tissue



    Lanes contain ~5 µg of total protein from Zea mays seedling leaves (upper image), Arabidopsis thaliana seedling leaves (lower image), or isolated chloroplast stroma. Leaf extract from homozygous pab mutants (hypomorphic alleles), and purified recombinant antigen were analyzed as controls. Proteins were separated on 12% SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked for 45 min at room temperature (RT) in TBST +4% dry milk with agitation. The blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed twice in TBST, then washed three times for 10 min in TBST at RT with agitation. The blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in TBST +1% dry milk for 1h at RT with agitation. The blot was washed as above and incubated for ~2 minutes in ECL reagents prior to imaging with a LiCor digital imager. Exposure time was ~30 seconds.

    Courtesy of Dr. Alison Barkan, The University of Oregon, USA
  • Background
  • Background: PAB (protein in chloroplast atpase biogenesis) acts as an assembly chaperone that functions downstream of chaperonin 60 in the assembly of chloroplast ATP synthase coupling factor 1.
  • Protocols


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compartment marker of chloroplast plastoglobules

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