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AGO set
Tag Antibody Set 

PGL35 | Plastoglobulin 35; FIB1a; FBN1a

AS06 116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Citrus reticulata, Gossypium histrutum | cellular [compartment marker] of chloroplast plastoglobules

PGL35 | Plastoglobulin 35; FIB1a; FBN1a in the group Antibodies for Plant/Algal  / Photosynthesis  / Biogenesis/architecture at Agrisera AB (Antibodies for research) (AS06 116)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

Recombinant Arabidopsis thaliana PGL35 protein O81439, At4g04020

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution 1 : 20 (IC), 1 : 1000-1 : 3000 (WB)
Expected | apparent MW

35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Citrus reticulata, Gossypium hirsutum cv. Deltapine 90
Predicted reactivity

Brassica napus, Brassica campestris, Capsicum annum

Not reactive in

Chlamydomonas reinhardtii, Pheodactylum tricornutum, Pisum sativum

Application examples

Additional information

Additional information Cellular [compartment marker] of chloroplast plastoglobules.
For IC samples were embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections were blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies were diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections were rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).

AtPGL35 is highly similar to Pisum sativum PG1

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Background

Background

Plastoglobules are lipoprotein particles which can be found in chloroplasts. They are generally believed to have a function of lipid storage. Recent data suggest that plastoglobules can be also metabolically active, taking part in tocopherol synthesis and likely other pathways. Immunogen: Alternative name AtPap1, fibrillin-1, probable plastid-lipid-associated protein 1.

Product citations

Selected references Luo et al. (2015). Distinct carotenoid and flavonoid accumulation in a spontaneous mutant of Ponkan (Citrus reticulata Blanco) results in yellowish fruit and enhanced postharvest resistance. J Agric Food Chem. 2015 Sep 2.
Gámez-Arjona et al. (2014). Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis. Plant J. 2014 Oct;80(2):305-16. doi: 10.1111/tpj.12633.

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