PGL35 | Plastoglobulin 35; FIB1a; FBN1a

Product no: AS06 116

AS06 116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Citrus reticulata, Gossypium histrutum

compartment marker of chloroplast plastoglobules

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  • Product Info
  • Immunogen:

    Recombinant Arabidopsis thaliana PGL35 protein O81439, At4g04020

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocytochemistry (ICC), Western blot (WB)
    Recommended dilution: 1 : 20 (IC), 1 : 1000-1 : 3000 (WB)
    Expected | apparent MW:

    35 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Citrus reticulata, Gossypium hirsutum cv. Deltapine 90
    Predicted reactivity:

    Brassica napus, Brassica campestris, Capsicum annum, Hordeum vulgare


    Species of your interest not listed? Contact us
    Not reactive in:

    Chlamydomonas reinhardtii, Pheodactylum tricornutum, Pisum sativum

  • Additional Information
  • Additional information: Cellular [compartment marker] of chloroplast plastoglobules.
    For IC samples were embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections were blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies were diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections were rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).
    Additional information (application):

    AtPGL35 is highly similar to Pisum sativum PG1

  • Background
  • Background:

    Plastoglobules are lipoprotein particles which can be found in chloroplasts. They are generally believed to have a function of lipid storage. Recent data suggest that plastoglobules can be also metabolically active, taking part in tocopherol synthesis and likely other pathways. Immunogen: Alternative name AtPap1, fibrillin-1, probable plastid-lipid-associated protein 1.

  • Product Citations
  • Selected references: (2021). Autophagy is required for lipid homeostasis during dark-induced senescence.Plant Physiology, 2021;, kiaa120
    Luo et al. (2015). Distinct carotenoid and flavonoid accumulation in a spontaneous mutant of Ponkan (Citrus reticulata Blanco) results in yellowish fruit and enhanced postharvest resistance. J Agric Food Chem. 2015 Sep 2.
    Gámez-Arjona et al. (2014). Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis. Plant J. 2014 Oct;80(2):305-16. doi: 10.1111/tpj.12633.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    Chiung-Chih, Chu | 2012-04-24
    0 | 0
    The antidody is specifically recognized a ~30 kDa protein in Arabidopsis chloroplasts but not in pea (20 micrograms of protein). The dilution factor for PGL35 is 1:2000 and for 2nd Ab is 1:50000 (by ECL).

Accessories

AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

Benefits of using this antibody

302 €
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

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