Name:
Phone:
E-mail:
Address:


 


PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae)

AS21 4552 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Saccharomyces cerevisiae

PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4552)

DATA SHEET IN PDF

Qty: 
320
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant PCNA protein from Saccharomyces cerevisiae, UniProt: P15873
Host Rabbit
Clonality Polyclonal
Purity Total immunoglobulin fraction
Format Liquid in PBS with 50 % glycerol, filter sterilized
Quantity 100 µg
Storage Store at -20°C and avoid temperature below -25°C ; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Inmunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 100 (IP), 1 : 1000 (WB)
Expected | apparent MW 28.9 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-PCNA antibodies


20 µg of a crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane, followed by blotting in 5 % non-fat milk for 1h/RT. Primary antibody was incubated at 1: 1000 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Related products

Related products AS21 4549 | Anti-RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade), rabbit antibodies

Background

Background This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's accessibility during elongation of the leading strand. Involved in DNA repair and recombination.

Product citations

Selected references Ogiwara et al. (2007). The INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell Cycle. 2007 May 2;6(9):1090-5. doi: 10.4161/cc.6.9.4130. Epub 2007 May 8. PMID: 17471029. (ChIP)
Hishida et al (2006). Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006 Jul;26(14):5509-17. doi: 10.1128/MCB.00307-06. PMID: 16809783; PMCID: PMC1592726. (Western Blot)
Iida et al.(2002). PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007. doi: 10.1046/j.1365-2443.2002.00584.x. PMID: 12354094. (Immunoprecipitation)

Related products: PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS21 4549  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Sacchar...
320 €
AS21 4550  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Saccharomyce...
320 €