PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae)
AS21 4552 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae
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Product Information
Immunogen
Recombinant PCNA protein from Saccharomyces cerevisiae, UniProt: P15873
Host
Rabbit
Clonality
Polyclonal
Purity
Total immunoglobulin fraction
Format
Liquid in PBS with 50 % glycerol, filter sterilized
Quantity
100 µg
Storage
Store at -20°C and avoid temperature below -25°C ; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP), Inmunoprecipitation (IP), Western blot (WB)
Recommended dilution
1: 100 (IP), 1 : 1000 (WB)
Expected | apparent MW
28.9 kDa
Reactivity
Confirmed reactivity
Saccharomyces cerevisiae
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples
20 µg of a crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane, followed by blotting in 5 % non-fat milk for 1h/RT. Primary antibody was incubated at 1: 1000 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
20 µg of a crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane, followed by blotting in 5 % non-fat milk for 1h/RT. Primary antibody was incubated at 1: 1000 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Additional information
Background
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's accessibility during elongation of the leading strand. Involved in DNA repair and recombination.
Product citations
Selected references
Ogiwara et al. (2007). The INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell Cycle. 2007 May 2;6(9):1090-5. doi: 10.4161/cc.6.9.4130. Epub 2007 May 8. PMID: 17471029. (ChIP)
Hishida et al (2006). Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006 Jul;26(14):5509-17. doi: 10.1128/MCB.00307-06. PMID: 16809783; PMCID: PMC1592726. (Western Blot)
Iida et al.(2002). PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007. doi: 10.1046/j.1365-2443.2002.00584.x. PMID: 12354094. (Immunoprecipitation)
Hishida et al (2006). Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006 Jul;26(14):5509-17. doi: 10.1128/MCB.00307-06. PMID: 16809783; PMCID: PMC1592726. (Western Blot)
Iida et al.(2002). PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007. doi: 10.1046/j.1365-2443.2002.00584.x. PMID: 12354094. (Immunoprecipitation)
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