PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae)

Product no: AS21 4552

AS21 4552 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Saccharomyces cerevisiae

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  • Product Info
  • Immunogen: Recombinant PCNA protein from Saccharomyces cerevisiae, UniProt: P15873
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total immunoglobulin fraction in PBS. Contains 50 % glycerol, filter sterilized.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C and avoid temperature below -25C ; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin immunoprecipitation (ChIP), Inmunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1: 100 (IP), 1 : 1000 (WB)
    Expected | apparent MW: 28,9 kDa
  • Reactivity
  • Confirmed reactivity: Saccharomyces cerevisiae
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Western blot using anti-PCNA antibodies

    20 µg of a crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane, followed by blotting in 5 % non-fat milk for 1h/RT. Primary antibody was incubated at 1: 1000 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
  • Background
  • Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's accessibility during elongation of the leading strand. Involved in DNA repair and recombination.
  • Product Citations
  • Selected references: Ogiwara et al. (2007). The INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell Cycle. 2007 May 2;6(9):1090-5. doi: 10.4161/cc.6.9.4130. Epub 2007 May 8. PMID: 17471029. (ChIP)
    Hishida et al (2006). Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006 Jul;26(14):5509-17. doi: 10.1128/MCB.00307-06. PMID: 16809783; PMCID: PMC1592726. (Western Blot)
    Iida et al.(2002). PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007. doi: 10.1046/j.1365-2443.2002.00584.x. PMID: 12354094. (Immunoprecipitation)
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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