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PEC1 | Plastid Envelope Channel 1

AS20 4528   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

compartment marker of chloroplast inner envelope membrane

PEC1 | Plastid Envelope Channel  1 in the group Antibodies for Plant/Algal  / Compartment Markers / Chloroplast marker / Chloroplast inner envelope membrane at Agrisera AB (Antibodies for research) (AS21 4528)

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Product Information

Immunogen The soluble domain 466 (M244 until stop codon, ≈ 64 kDa) was cloned into pET16b and transformed into BLR 21 for 467 expression in Escherichia coli UniProt: Q8VZM7-1, TAIR: At5g02940
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 100 | 75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Camelina sativa, Capsella rubella, Nicotiana benthamiana, Noccaea caerulescens, Pisum sativum, Raphanus sativus,

Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-PEC1 antibodies
Samples:
1 – 2,5 ug of Arabidopsis thaliana mutant pec1-1pec2-1
2 – 2,5 ug of Arabidopsis thaliana mutant pec1-2pec2-2
3 – 2,5 ug of Arabidopsis thaliana whole leaf extract

Arabidopsis thaliana tissue was frozen with liquid nitrogen and ground to a fine powder with mortar and pestle. Protein was extracted by mixing with extraction buffer (200 mM tris pH 8.0, 4% (w/v) sodium dodecyl sulfate) to 0.5 g fresh weight/mL and heating at 80°C for 8 min. 5 µl protein extract were separated on 10 % SDS-PAGE and blotted at 75v for 45min to nitrocellulose (pore size of 20 um), using wet transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 ON/4°C in 5% Milk-TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in  matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 1 min with chemiluminescent detection reagent following manufacture's recommendations. Exposure time was 120 seconds.

Courtesy of Carsten Voelkner, Kunz Lab

Additional information

Additional information This antibody is recognizing PEC1, but not PEC2 or DMI1.

Related products

Background

Background The two Arabidopsis POLLUX-like homologs PEC1 and PEC2 represent plastid inner 31 envelope membrane cation channels with K + conductivity that are required for the stress- 32 triggered Ca 2+ release into the stroma.

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