P-Tyr | Phosphotyrosine (clone G104)
AS10 705 | Clonality: monoclonal | Host: Mouse | Reactivity: phosphotyrosine

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Total IgG.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Reactivity
Confirmed reactivity
Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Protein G purified IgG1 in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
1 ĩg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 ĩg of rat tissue lysate by colorimetric immunoblot analysis
Background
Background
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
Product citations
Selected references
Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):
Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):
Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
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