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P-Tyr | Phosphotyrosine (clone G104)

AS10 705   | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: phosphotyrosine

P-Tyr | Phosphotyrosine (clone G104) in the group Antibodies Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS10 705)
P-Tyr | Phosphotyrosine (clone G104)



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Product Information

Immunogen

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Total IgG.
Format Liquid
Quantity 100 ĩg
Storage Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000 (WB)

Reactivity

Confirmed reactivity Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Protein G purified IgG1 in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
1 ĩg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 ĩg of rat tissue lysate by colorimetric immunoblot analysis

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Background

Background

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.

Product citations

Selected references Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):
Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):

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