PHOT1 | Phototropin-1

AS10 720 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PHOT1 | Phototropin-1 in the group Antibodies for Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS10 720)


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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, At3g45780

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

111 | 132 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in Cuscuta campestris, Oryza sativa

Application examples

Application examples application example
western blot using anti-PHOT1 antibodies
80 µg of total protein from Arabidopsis thaliana wt Columbia (right lane) and Arabidopsis thaliana phot1phot2 double mutant (left lane) were separated on 9% SDS-PAGE and blotted 2h to PVDF. Blots were blocked immediately following transfer in PBS-T 5% milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5000 overnight at 4oC with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at room temperature with agitation. The blots were washed as above and developed with WestPico detection reagent (PIERCE) according to the manufacturers instructions. Exposure time was 300 seconds.

Additional information

Additional information This product can be sold containing ProClin if requested
Information about phot1 mutant, first named nph1:
  • Liscum &. Briggs (1995). Mutations in the NPH1 Locus of Arabidopsis Disrupt the Perception of Phototropic Stimuli. The Plant Cell, Vol. 7, 473-485.
  • Huala et al. (1997). Arabidopsis NPH1: A Protein Kinase with a Putative Redox-Sensing Domain. Science 19: Vol. 278 no. 5346 pp. 2120-2123.
  • Lehmann et al. (2011). Transitions of gene expression induced by short-term blue light. Plant Biology Volume 13, Issue 2, pages 349–361. Seeds of this mutant are available at uNASC.

Related products

Related products

AS10 721 | PHOT2 | phototropin-2

Plant protein extraction buffer

Secondary antibodies



PHOT1 | phototropin-1 is a blue-light photoreceptor which containst light activated serine-threonine kinase domain. Is required for stomatal opening, chloroplast movements, leaf flattening and phototropism. Undergoes blue-light-dependent autophosphorylation. Alternative names: NPH1, RPT1, JK224, Non-phototropic hypocotyl protein 1, Root phototropism protein 1

Product citations

Selected references Labuz et al. (2021) Phototropin interactions with SUMO proteins. Plant Cell Physiol. 2021 Feb 17:pcab027. doi: 10.1093/pcp/pcab027. Epub ahead of print. PMID: 33594440.
Krzeszowiec et al. (2020). Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Rep . 2020 Oct;39(10):1331-1343.doi: 10.1007/s00299-020-02567-3. Epub 2020 Jul 13.
Labuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.
Eckstein et al. (2015). Auxin and chloroplast movements. Physiol Plant. 2015 Oct 15. doi: 10.1111/ppl.12396.

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