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PHOT2 | Phototropin-2

AS10 721 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PHOT2 | Phototropin-2 in the group Antibodies for Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS10 721)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, At5g58140

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

102 | 110 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in

Oryza sativa

Application examples

Application examples

application example

80 µg of total protein from Arabidopsis thaliana wt Columbia (right lane) and Arabidopsis thaliana phot1phot2 double mutant (left lane) were separated on 9% SDS-PAGE and blotted 2h to PVDF. Blots were blocked immediately following transfer in PBS-T 5% milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 overnight at 4oC with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti- rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at room temperature with agitation. The blots were washed as above and developed with WestPico detection reagent (PIERCE) according to the manufacturers instructions. Exposure time was 300 seconds.

 


western blot using anti-AtPHOT2 antibodies

Additional information

Additional information

This product can be sold containing ProClin if requested.

Related products

Related products

AS10 720 | PHOT1 | phototropin-1

Plant protein extraction buffer

Secondary antibodies

Background

Background

PHOT2 | phototropin-2 is a membrane-bound serine/threonine kinase that functions as blue light photoreceptor in redundancy with PHOT1. Involved in processed like stomatal opening, chloroplast movement and phototropism. Alternative names: NPL1, K21L19.6, AtKin7, Defective in chloroplast avoidance protein 1
Non-phototropic hypocotyl 1-like protein 1, NPH1-like protein 1.

Product citations

Selected references Krzeszowiec et al. (2020). Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Rep . 2020 Oct;39(10):1331-1343.doi: 10.1007/s00299-020-02567-3. Epub 2020 Jul 13.
Labuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.
Aggarwal et al. (2014). Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles. J Exp Bot. 2014 May 12.

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AS10 720 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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