Phy | Phytochrome (clone Pea-25)

Name: Phy | Phytochrome (clone Pea-25)
Brand: IKP
SKU: AS20 4502
Price: 331 EUR
Availability: InStock

AS20 4502 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum

Phy | Phytochrome (clone Pea-25) in the group Antibodies Plant/Algal / Plant Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS20 4502)

Product Information

Immunogen Phytochrome

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG

Purity Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Competitive ELISA, Immunoprecipiation (IP), Western blot (WB)

Recommended dilution assay dependent

Expected | apparent MW 124 kDa

Reactivity

Confirmed reactivity Avena sativa, Pisum sativum

Predicted reactivity graminae, fabaceae
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Epitope recognized by this antibody is located at 85 kDa from N-terminus and is highly conserved (no photo-conversion)

Background

Background Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.

Product citations

Selected references Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1.
Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.

immunogen:	Phytochrome
Sub class:	IgG
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.
Format:	Liquid
Quantity:	100 µg
storage:	Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Competitive ELISA, Immunoprecipiation (IP), Western blot (WB)
recommended dilution:	assay dependent
calculated \| apparent molecular mass [kDa]:	124 kDa
Confirmed reactivity:	Avena sativa, Pisum sativum
predicted reactivity:	graminae, fabaceae Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	Epitope recognized by this antibody is located at 85 kDa from N-terminus and is highly conserved (no photo-conversion)
background:	Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.
All references:	Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1. Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.