PIF4 | Phytochrome interacting factor 4 (rabbit antibody)
AS16 3157 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Immunogen
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin Immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution
1: 1000 (WB)
Expected | apparent MW
48,3 | 60 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example

5-20 µg of Arabidopsis thaliana nuclear proteins were extracted according to Gendrel et al. 2005. Samples were denatured with SDS page loading buffer at 90°C for 5 min, separated on 12% SDS-PAGE and then blotted 1h to PVDF using wet transfer. Blot was blocked with 5% milk 1h at RT with agitation and then It was incubated with the anti-PIF4 antibody at a dilution of 1:1000 in TBS-T ON at 4°C in agitation. The antibody solution was decanted and the blot was rinsed briefly with TBS-T, then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in TBS-T for 1h/RT with agitation (Agrisera AS09 602). The blot was washed as above and developed with chemiluminescent ragent. Exposure time was 30 min.
Courtesy of Dr. Lucia Ferrero, Universidad Nacional del Litoral, Argentina

5-20 µg of Arabidopsis thaliana nuclear proteins were extracted according to Gendrel et al. 2005. Samples were denatured with SDS page loading buffer at 90°C for 5 min, separated on 12% SDS-PAGE and then blotted 1h to PVDF using wet transfer. Blot was blocked with 5% milk 1h at RT with agitation and then It was incubated with the anti-PIF4 antibody at a dilution of 1:1000 in TBS-T ON at 4°C in agitation. The antibody solution was decanted and the blot was rinsed briefly with TBS-T, then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in TBS-T for 1h/RT with agitation (Agrisera AS09 602). The blot was washed as above and developed with chemiluminescent ragent. Exposure time was 30 min.
Courtesy of Dr. Lucia Ferrero, Universidad Nacional del Litoral, Argentina
Additional information
Additional information
PIF proteins are very unstable, therefore special precautions should be taken during extraction and whole procedure should be performed in the dark and with as little light as possible (green light only).
Extraction of PIF proteins is described in Shen et al. (2007).
This product can be sold containing ProClin if requested.
Background
Background
PIF4 (PHYTOCHROME INTERACTING FACTOR 4) is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.
Product citations
Selected references
Gras et al. (2020). Arabidopsis Thaliana SURFEIT1-like Genes Link Mitochondrial Function to Early Plant Development and Hormonal Growth Responses. Plant J . 2020 Apr 5. doi: 10.1111/tpj.14762.
Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.
Hwang et al. (2019). Trehalose-6-phosphate signaling regulates thermoresponsive hypocotyl growth in Arabidopsis thaliana. EMBO Rep. 2019 Aug 8:e47828. doi: 10.15252/embr.201947828.
Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.
Hwang et al. (2019). Trehalose-6-phosphate signaling regulates thermoresponsive hypocotyl growth in Arabidopsis thaliana. EMBO Rep. 2019 Aug 8:e47828. doi: 10.15252/embr.201947828.
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