PIF4 | Phytochrome interacting factor 4 (rabbit antibody)

Product no: AS16 3157

AS16 3157  |  Clonality: Polyclonal   |  Host: Rabbit   |  Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:
    KLH-conjugated peptide derived from Arabidopsis thaliana PIF4, UniProt: Q8W2F3, TAIR:AT2G43010
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin Immunoprecipitation (ChIP), Western blot (WB)
    Recommended dilution: 1: 1000 (WB)
    Expected | apparent MW: 48,3 | 60 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-PIF4 rabbit antibodies

    5-20 µg of Arabidopsis thaliana nuclear proteins were extracted according to Gendrel et al. 2005. Samples were denatured with SDS page loading buffer at 90°C for 5 min, separated on 12% SDS-PAGE and then blotted 1h to PVDF using wet transfer. Blot was blocked with 5% milk 1h at RT with agitation and then It was incubated with the anti-PIF4 antibody at a dilution of 1:1000 in TBS-T ON at 4°C in agitation. The antibody solution was decanted and the blot was rinsed briefly with TBS-T, then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in TBS-T for 1h/RT with agitation (Agrisera AS09 602). The blot was washed as above and developed with chemiluminescent ragent. Exposure time was 30 min.

    Courtesy of Dr. Lucia Ferrero, Universidad Nacional del Litoral, Argentina
  • Additional Information
  • Additional information:

    PIF proteins are very unstable, therefore special precautions should be taken during extraction and whole procedure should be performed in the dark and with as little light as possible (green light only).

    Extraction of PIF proteins is described in Shen et al. (2007).

    This product can be sold containing ProClin if requested.

  • Background
  • Background:
    PIF4 (PHYTOCHROME INTERACTING FACTOR 4) is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.
  • Product Citations
  • Selected references: Gras et al. (2020). Arabidopsis Thaliana SURFEIT1-like Genes Link Mitochondrial Function to Early Plant Development and Hormonal Growth Responses. Plant J . 2020 Apr 5. doi: 10.1111/tpj.14762.
    Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.
    Hwang et al. (2019). Trehalose-6-phosphate signaling regulates thermoresponsive hypocotyl growth in Arabidopsis thaliana. EMBO Rep. 2019 Aug 8:e47828. doi: 10.15252/embr.201947828.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (1)
    Vasundara Sundar | 2022-12-28
    0 | 0
    I have used this antibody for western blotting at 1:1000 dilution. The results were good with high specificity.


AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart

AS16 3955 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
img missing
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 17 €