IKP

Plant Vitamin A ELISA quantitation kit

Product no: AS15 3024

AS15 3024 | Reactivity: Plant Vitamin A

1019
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Delivery:  3-6 business days
  • Data sheet
  • Background | This ELISA immunoassay utilize the principle of competitive inhibition for quantitative determination of endogenous Vitamin A (VA) concentrations in plant tissues.

    An antibody specific to VA is pre-coated on the surface of a micro titer plate. Standards or samples are added to the plate wells including VA conjugated to HRP. The reaction of competitive inhibition is initiated between the HRP conjugated Vitamin A and Vitamin A in the samples. A substrate solution is then added and the color develops which is inversely proportional to the amount of Vitamin A in the sample. The intensity of the color is measured after the reaction is stopped.


    This Plant Vitamin A ELISA kit contains the following reagents:
    Assay plate: 1x (96 wells)
    Standards: 1 x 200 µl (10 x concentrate)
    Sample diluent: 2 x 20 ml
    HRP-conjugate: 1 x 120 µl (100 x concentrate)
    HRP-conjugate Diluent: 1 x 10 ml
    Washing Buffer: 1 x 20 ml (25 x concentrate)
    TMB Substrate: 1 x 10 ml (store in the dark)
    Stop Solution: 1 x 10 ml (contains acid, wear eye, hand, face and clothing protection)
    Adhesive Strip: 4x (for 96 wells)


    This kit is for research purpose only.

    ________________________________________________________________________________________

    Storage: Store at 2-8°C. Opened kit can be stored at 2-8°C for maximum of 1 month.

    Expiry date:
    Kit should not be used beyond expiry date.

    Detection range:
    0.625-10 ng/ml

    Specificity/Sensitivity:
    High specificity and sensitivity for plant Vitamin A. No significant cross reactivity has been observed.


    Manual in Pdf

    • Product Citations
    • Selected references: 38221900
  • Protocols
  • Protocols and information
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