Plant Vitamin A ELISA quantitation kit
AS15 3024 | Reactivity: Plant Vitamin A
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Background | This ELISA immunoassay utilize the principle of competitive inhibition for quantitative determination of endogenous Vitamin A (VA) concentrations in plant tissues.
An antibody specific to VA is pre-coated on the surface of a micro titer plate. Standards or samples are added to the plate wells including VA conjugated to HRP. The reaction of competitive inhibition is initiated between the HRP conjugated Vitamin A and Vitamin A in the samples. A substrate solution is then added and the color develops which is inversely proportional to the amount of Vitamin A in the sample. The intensity of the color is measured after the reaction is stopped.
This Plant Vitamin A ELISA kit contains the following reagents:
Assay plate: 1x (96 wells)
Standards: 1 x 200 µl (10 x concentrate)
Sample diluent: 2 x 20 ml
HRP-conjugate: 1 x 120 µl (100 x concentrate)
HRP-conjugate Diluent: 1 x 10 ml
Washing Buffer: 1 x 20 ml (25 x concentrate)
TMB Substrate: 1 x 10 ml (store in the dark)
Stop Solution: 1 x 10 ml (contains acid, wear eye, hand, face and clothing protection)
Adhesive Strip: 4x (for 96 wells)
This kit is for research purpose only.
Storage: Store at 2-8°C. Opened kit can be stored at 2-8°C for maximum of 1 month.
Expiry date: Kit should not be used beyond expiry date.
Detection range: 0.625-10 ng/ml
Specificity/Sensitivity: High specificity and sensitivity for plant Vitamin A. No significant cross reactivity has been observed.