Rubisco ELISA quantitation kit

Background | This ELISA immunoassay utilize the principle of competitive inhibition for quantitative determination of endogenous Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in plant tissues.

Rubisco antigen is pre-coated on the surface of the micro titer plate. Standards or samples are added to the plate wells including an antibody specific for Rubisco. The reaction of competitive inhibition is initiated between the pre-coated Rubisco and Rubisco in the samples with the antibody. A goat anti-rabbit IgG, HRP-conjugated, antibody is added followed by a substrate solution and the color develops which is inversely proportional to the amount of Rubisco in the sample. The intensity of the color is measured after reaction is is   stopped.


This Rubisco ELISA kit contains the following reagents:
Assay plate: 1x (96 wells)
Standards: 2 x 500 µl
Antibody: 1 x 60 µl (100 x concentrate)
HRP-conjugate: 1 x 120 µl (100 x concentrate)
Antibody Diluent: 1 x 10 ml
HRP-conjugate Diluent: 1 x 20 ml
Sample Diluent: 2 x 20 ml
Sample Extraction Buffer: 1 x 20 ml (25 x concentrate)
Washing Buffer: 1 x 20 ml (25 x concentrate)
TMB Substrate: 1 x 10 ml (store in the dark)
Stop Solution: 1 x 10 ml (contains acid, wear eye, hand, face and clothing protection)
Adhesive Strip: 4x (for 96 wells)

This kit is for research purpose only. Proclin 300 is used as preservative, in concentration <1 %. 

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Storage: Store at 2-8°C. Opened kit can be stored at 2-8°C for maximum of 1 month.

Expiry date: Kit should not be used beyond expiry date.

Detection range: 3.12-800 µg/ml

Specificity/Sensitivity: High specificity and sensitivity for plant Rubisco. No significant cross reactivity has been observed.