AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11

AS10 920 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11 in the group Plant/Algal Antibodies / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS10 920)


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product information

AKIN11 (E.C.= is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AKIN11 sequence P92958, At3g29160

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 25 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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AS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibody
AS09 463 | Anti-AKIN beta gamma, rabbit antibody
AS10 919 | Anti-AKIN10  SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibody
AS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibody

collection of antibodies to proteins involved in signal transduction

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

58.6 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in

Oryza sativa, Vitis vinfera

Additional information
Selected references Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.

application example

western blot with anti-AKIN11 antibodies

Total protein from 20 000 Arabidopsis thaliana leaf mesophyll protoplasts transiently expressing HA-tagged KIN10 or KIN11 (or transfected with control plasmid, C) were separated on 10% SDS-PAGE  and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat milk in TBST (0.05% Tween20) for 1h at room temperature (RT) with agitation. Blot was incubated in anti-HA (left) or anti-KIN11 (right) at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and exposed to film, developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was  60 seconds.

Courtesy Dr. Filip Rolland, KU Leuven, Belgium

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