PR-3 / CHN | Class I chitinase
AS07 207 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. stolonifera cv. ‘Penncross’, C. annuum, N. tabacum, P. abies, S. esculentum, S. lycopersicum, S. tuberosum, V. vinifera | Cellular marker of vacuolar contents
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|Recommended dilution||8 ug/ml (WB)|
|Expected | apparent MW||
35, 34 | 32 and 34 kDa
|Confirmed reactivity||Agostis stolonifera cv. ‘Penncross’, Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
|Predicted reactivity||Arabidopsis thaliana, Zea mays|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.
This antibody is purified by affinity chromarography on Portein G.
|Selected references||Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Ko et al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.
Anil Kumar et al. (2016). Beyond just being foot soldiers – osmotin like protein (OLP) and chitinase (Chi11) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato. Environmental and Experimental Botany 132 (2016) 53–65
Wu et al. (2016). Laminarin modulates the chloroplast antioxidant system to enhance abiotic stress tolerance partially through the regulation of the defensin-like gene expression. Plant Science, Volume 247, June 2016, Pages 83–92.
Falcioni et al. (2013). Effect of salicylic acid treatment on tomato plant physiology and tolerance to potato virus X infection. Eur J Plant Pathol DOI 10.1007/s10658-013-0333-1.
Munger et al. (2012). Beneficial 'unintended effects' of cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology, 12:198.
Sticher et al. (1993). Posttranslational processing of a new class of hydroxyproline-containing proteins: Prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase. Plant Physiol. 101, 1239-1247.
Detection of tobacco chitinase I in ng loaded per respective well using anti-tobacco chitinase I antibodies. Primary antibodies have been used at 8 µg/ml.
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