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Tag Antibody Set 

PR-2 | GLU I | Class I beta-1,3-glucanase

AS07 208  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: N. benthamiana, N. clevilandii, N. tabacum, Phalenopsis, P.abies, S.lycopersicum, S. tuberosum, V. vinifera | compartment marker of vacuolar contents

PR-2 | GLU I | Class I beta-1,3-glucanase in the group Antibodies for Plant/Algal  / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS07 208)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

Purified tobacco class I, basic  ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

Host Rabbit
Clonality Polyclonal
Purity Total IgG in PBS pH 7.4 (without Ca++).
Format Lyophilized
Quantity 2 mg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 8 µg/ml (WB)
Expected | apparent MW

37 | 33 kDa

Reactivity

Confirmed reactivity Fragaria vesca, Lactuca sativa, Nicotiana benthamian, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Predicted reactivity Dicots, Oryza sativa, Prunus persica
Not reactive in

Arabidopsis thaliana 

Application examples

Application examples

Application example

western blot detection of tobacco class I beta 1,3 glucanase

Detection of tobacco tobacco class I ß 1,3 – glucanase in ng loaded per respective well using anti- tobacco class I ß 1,3 – glucanase antibodies. Primary antibodies have been used at 8 µg/ml.

Additional information

Additional information

For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51.

This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51. 

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.

This antibody is purified by affinity chromarography on Portein G.

Related products

Related products

AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies

AS12 2366 | Anti-PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thaliana

AS07 207
| Anti-PR-3 | CHN | class I chitinase, rabbit antibodies

AS12 2369 | Anti-PR-4 | Pathogenesis-related protein 4, rabbit antibodies

AS12 2373 | Anti-PR-5 | Pathogenesis-related protein 5, rabbit antibodies

collection of antibodies to other proteins involved in a response to pathogen attack

Secondary antibodies

Background

Background

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

Product citations

Selected references Martin-Saladana et al. (2018). Salicylic acid loaded chitosan microparticles applied to lettuce seedlings: Recycling shrimp fishing industry waste. Carbohydrate Polymers Volume 200, 15 November 2018, Pages 321-331.
Wang et al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201.
Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.
Munger et al. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.

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