NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic)
AS16 4064 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
|Info:||Product suggestions||Add review|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Zea mays|
|Predicted reactivity||Anastatica hierochuntica, Arabis stelleri, Barbarea verna, Braya humilis, Bunias orientalis, Cakile arabica, Camelina sativa, Cannabis sativa, Capsella bursa-pastoris, Capsella rubella, Cardamine impatiens, Chorispora tenella, Cleomella serrulata, Cochlearia pyrenaica, Crucihimalaya wallichii, Dontostemon micranthus, Euclidium syriacum, Eutrema yunnanense, Farsetia stylosa, Fragaria ananassa, Hesperis matronalis, Ionopsidium acaule, Lepidium virginicum, Lobularia maritima, Megadenia pygmaea, Matthiola incana, Nasturtium officinale, Neotorularia korolkowii, Noccaea caerulescens, Olimarabidopsis pumila, Orychophragmus taibaiensis, Pachycladon enysii, Physaria ludoviciana, Pugionium cornutum, Schrenkiella parvula, Tarenaya hassleriana, Thlaspi arvense|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
To be added when available, antibody released in November 2017.
5µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 mM Tris pH7.5, 10% glycerol, 1 mM EDTA, 1mM EGTA, 2mM pMSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 mM DTT) and denatured in 1XPSB (33 mM Tris pH 6.8, 50 mM 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70C for 5 min, separated on 4-20% Tris-Glycine SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked with 10% milk in 1XTBSt for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation in 10% milk in 1XTBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10,000 in 10% milk in 1XTBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent.
Courtesy Rosalind Carrier, University of Oregon, USA
||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at firstname.lastname@example.org