Name:
Phone:
E-mail:
Message

Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

Pre-apoplastocyanin

AS06 118  | Clonality: Polyclonal  | Host: Rabbit  | Reactivity: Chlamydomonas reinhardtii

Pre-apoplastocyanin in the group Plant/Algal Antibodies / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS06 118)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

Plastocyanin is a “blue” copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.

Immunogen

GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii  P18068

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS06 141 | anti-plastocyanin

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

This product can be sold containing ProClin if requested.

application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17 kDa (precursor)

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlorella fusca, Pediastrum boryanum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.

Selected references Li et al.  (1996).  Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com