Agrisera Super Deal


Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

AOX1 | Algal Alternative oxidase 1

AS06 152  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane

AOX1 | Algal Alternative oxidase 1 in the group Plant/Algal Antibodies / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS06 152)


355 €

Info: Add review
product information

The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The  alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport.  Alternative oxidase expression is  influenced by different stress stimuli.


whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii O65000 fused to GST

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS04 054 | anti-plant alternative oxidase 1 and 2 polyclonal antibody

AS10 699 | AOX | alternative oxidase monoclonal antibody

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

36 | 36 kDa (Chlamydomonas reainhardii)

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.
Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.

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