Name:
Phone:
E-mail:
Message

SHMT | Serine hydroxymethyltransferase

AS05 075  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  A. thaliana, P. sativum, S. oleracea, V. faba  |  cellular[compartment marker] of mitochondrial matrix

SHMT | Serine hydroxymethyltransferase in the group Plant/Algal Antibodies / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS05 075)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Read reviews
product information
Background

Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.  

Immunogen

Purified SHMT protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity

0.5 mg

Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS05 074 | anti-GDC-H rabbit antibody, marker of mitochondrial matrix

AS06 203A | anti- Idh | isocytrate dehydrogenase rabbit antibody, marker of mitochondrial matrix

AS07 212 | anti-VDAC1 marker antibody for mitochondrial outer membrane

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 1000 to 1 : 5 000 (WB)
Expected | apparent MW

53 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea, Oryza sativa, Pisum sativum, Spinacia oleracea, Vicia faba
Predicted reactivity Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Medicago truncatula, Populus balsamifera, Ricinus communis, Solanum tuberosum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody can be used on total leaf extracts and isolated mitochondria

Selected references Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins, Int. J. Mol. Sci. 2018, 19(4), 1130; doi:10.3390/ijms19041130 (Special issue: Plant Mitochondria)
Rurek et al. (2018). Cold and Heat Stress Diversely Alter Both Cauliflower Respiration and Distinct Mitochondrial Proteins Including OXPHOS Components and Matrix Enzymes, Int. J. Mol. Sci. 2018, 19(3), 877; doi:10.3390/ijms19030877 (Special issue: Plant Mitochondria)
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.
Camejo et al. (2012).Salinity-inducedchanges inS-nitrosylation ofpeamitochondrialproteins. J. Proteomics Dec.11.

Application example

Western blot using anti-SHMT antibodies


30 µg of total protein from cauliflower Brassica oleracea var. botrytis mitochondrial fraction, extracted according to Rurek el al. 2015 and denatured with standard 1 x conc. Sample Buffer with 2-mercaptoethanol at 80°C for 10 min were separated on 12 % SDS-PAGE and blotted 1h to Immobilon-P using semi-dry transfer in the standard transfer buffer (20 % methanol, 48 mM Tris, 39 mM glycine, 0.0375 % SDS). Blots were blocked with 5 % skimmed milk in PBS-T with 0.01 % Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in 2 % skimmed milk in PBS-T in 2 % skimmed milk at a dilution of 1: 10 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera ) diluted to 1:10 000 in PBS-T with 2 % milk, for 1h at RT with agitation. The blot was washed as above and developed for min with enhanced chemiluminescence. Exposure time was 5 s.

Courtesy of Michal Rurek, D.Sc., Adam Mickiewicz University, Poznań (Poland)

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com