SHMT | Serine hydroxymethyltransferase

AS05 075 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Citrus sp., P. sativum, S. oleracea, V. faba | cellular[compartment marker] of mitochondrial matrix

Product Information

Immunogen

Purified SHMT protein from Spinacia oleracea

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS pH 7.4.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 1000 to 1 : 5 000 (WB)

Expected | apparent MW

53 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea, Citrus sp., Oryza sativa, Pisum sativum, Spinacia oleracea, Vicia faba

Predicted reactivity Chlamydomonas reinhardii, Citrus unshiu, Glycine max, Hordeum vulgare, Medicago truncatula, Populus balsamifera, Ricinus communis, Solanum tuberosum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

30 µg of total protein from cauliflower Brassica oleracea var. botrytis mitochondrial fraction, extracted according to Rurek el al. 2015 and denatured with standard 1 x conc. Sample Buffer with 2-mercaptoethanol at 80°C for 10 min were separated on 12 % SDS-PAGE and blotted 1h to Immobilon-P using semi-dry transfer in the standard transfer buffer (20 % methanol, 48 mM Tris, 39 mM glycine, 0.0375 % SDS). Blots were blocked with 5 % skimmed milk in PBS-T with 0.01 % Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in 2 % skimmed milk in PBS-T in 2 % skimmed milk at a dilution of 1: 10 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera ) diluted to 1:10 000 in PBS-T with 2 % milk, for 1h at RT with agitation. The blot was washed as above and developed for min with enhanced chemiluminescence. Exposure time was 5 s.

Courtesy of Michal Rurek, D.Sc., Adam Mickiewicz University, Poznań (Poland)

Reactant: Oryza sativa (Asian rice)

Application: Western Blotting

Pudmed ID: 27124767

Journal: PLoS One

Figure Number: 6A

Published Date: 2016-04-29

First Author: Yin, G., Whelan, J., et al.

Impact Factor: 2.942

Open Publication

Abundance of a variety of known mitochondrial proteins by the immunoblot analysis of mitochondria isolated from 0 d, 3 d, and 4 d aged rice embryos after 48 h imbibition.Total 10 ?g protein was separated by SDS gel electrophoresis and blotted to supported polyvinylidene difluoride, then probed with antibodies against beta subunit of ATP synthase (AtpB), serine hydroxymethyltransferase (SHMT), isocitrate dehydrogenase (IDH), voltage dependent anion channel 1 (VDAC1) and cytochrome c (Cyt c).

Additional information

This antibody can be used on total leaf extracts and isolated mitochondria

Related products

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AS07 212 | Anti-VDAC1 marker for mitochondrial outer membrane, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

Product citations

Selected references Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.
Guo et al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana,Biochemical and Biophysical Research Communications,Volume 578,2021,Pages 63-69,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2021.09.022.(https://www.sciencedirect.com/science/article/pii/S0006291X21013164)
Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins, Int. J. Mol. Sci. 2018, 19(4), 1130; doi:10.3390/ijms19041130 (Special issue: Plant Mitochondria)
Rurek et al. (2018). Cold and Heat Stress Diversely Alter Both Cauliflower Respiration and Distinct Mitochondrial Proteins Including OXPHOS Components and Matrix Enzymes, Int. J. Mol. Sci. 2018, 19(3), 877; doi:10.3390/ijms19030877 (Special issue: Plant Mitochondria)
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba ? L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.
Camejo et al. (2012).Salinity-inducedchanges inS-nitrosylation ofpeamitochondrialproteins. J. Proteomics Dec.11.