POL I | DNA Polymerase 1
AS21 4547 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli
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Samples: 10 ng of purified DNA polymerase 1 from E.coli (1), E. coli strain AB1157 extract prepared by sonication (2) were separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 2000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.
Samples: Purified DNA polymerase 1 ( 20 ng) (1) Purified DNA polymerase 1 (10 ng) (2) Crude lysate of E. coli strain AB1157 (10 μg) (3) The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by Western blot by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP (suitable for detection of immunoprecipiation products) was used.
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