POL I | DNA Polymerase 1

AS21 4547 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

Product Information

Immunogen Purified, full length, recombinant POL I protein from E.coli, UniProt: P00582

Host

Rabbit

Clonality Polyclonal

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 100 µl

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution assay dependent (IP), 1 : 1000 - 1: 5000 (WB)

Expected | apparent MW 103 kDa

Reactivity

Confirmed reactivity Escherichia coli

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-DNA polymerase I antibodies

Samples: 10 ng of purified DNA polymerase 1 from E.coli (1), E. coli strain AB1157 extract prepared by sonication (2) were separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 2000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.

Samples: Purified DNA polymerase 1 ( 20 ng) (1) Purified DNA polymerase 1 (10 ng) (2) Crude lysate of E. coli strain AB1157 (10 μg) (3) The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by Western blot by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP (suitable for detection of immunoprecipiation products) was used.

Additional information

Background

Background E. coli DNA polymerase 1 (928 aa; 103 kDa) is encoded by polA gene and involved in DNA replication and repair. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Product citations

Picture (footer):	Samples: 10 ng of purified DNA polymerase 1 from E.coli (1), E. coli strain AB1157 extract prepared by sonication (2) were separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 2000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations. Samples: Purified DNA polymerase 1 ( 20 ng) (1) Purified DNA polymerase 1 (10 ng) (2) Crude lysate of E. coli strain AB1157 (10 μg) (3) The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by Western blot by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP (suitable for detection of immunoprecipiation products) was used.
Confirmed reactivity:	Escherichia coli
predicted reactivity:	Species of your interest not listed? Contact us
calculated \| apparent molecular mass [kDa]:	103 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Purified, full length, recombinant POL I protein from E.coli, UniProt: P00582
Purity:	Serum. Contains 0.05 % sodium azide.
Quantity:	100 µl
recommended dilution:	assay dependent (IP), 1 : 1000 - 1: 5000 (WB)
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
background:	E. coli DNA polymerase 1 (928 aa; 103 kDa) is encoded by polA gene and involved in DNA replication and repair. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

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