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POL I | DNA Polymerase 1

AS21 4547 | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

POL I | DNA Polymerase 1 in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4547)

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Product Information

Immunogen Purified, full length, recombinant POL I protein from E.coli, UniProt: P00582
Host

Rabbit

Clonality Polyclonal
Purity Serum with 0.05 % sodium azide
Format Liquid
Quantity 100 µl
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution assay dependent (IP), 1 : 1000 - 1: 5000 (WB)
Expected | apparent MW 103 kDa

Reactivity

Confirmed reactivity Escherichia coli
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-DNA polymerase I antibodies

Samples: 10 ng of purified DNA polymerase 1 from E.coli (1),  E. coli strain AB1157 extract prepared by sonication (2) were separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 2000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.


Immunoprecipitation using anti-polymerase I antibodies


Samples: Purified DNA polymerase 1 ( 20 ng) (1) Purified DNA polymerase 1 (10 ng) (2) Crude lysate of E. coli strain AB1157 (10 μg) (3) The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by Western blot by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP (suitable for detection of immunoprecipiation products) was used.

Additional information

Related products

Background

Background E. coli DNA polymerase 1 (928 aa; 103 kDa) is encoded by polA gene and involved in DNA replication and repair. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Product citations

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