PPD2 | Protein PEAPOD 2

Product no: AS15 3107

AS15 3107 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PPD2 sequence, Uniprot: Q8GY55 TAIR: At4G14720
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Expected | apparent MW: 34,8 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Equal volumes of total proteins from E. coli purified with GST agarose beads or N. benthamiana were extracted and denatured with with Laemlli buffer (50 mM Tris pH 8, 10 mM EDTA, 18% glycerol, and 10 mM DTT) at 95°C for 10 min. Samples were separated on 12% SDS-PAGE and blotted 30 min to Protran nitrocellulose membrane (Whatman) using semi-dry transfer. Blots were blocked with 0.25% BSA/0.25% gelatin for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 4 times for 15 min in 10mM Tris pH8.0, 0.3% Tween 20 buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:10 000 in for 60 min at RT with agitation. The blot was washed as above and developed for 10 min with NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate; Promega Corporation).

    Courtesy of Dr. Garry Sunter, South Texas Center for Emerging Infectious Disease, San Antonio, USA
  • Additional Information
  • Additional information: This antibody is recognizing recombinant PPD2 and reactivity on endogenous protein needs to be confirmed
  • Background
  • Background:

    PPD2 (protein PEABOD 2) belongs to the TIFY/JAZ family and is involved in lamina development, regulating leaf size and its curvature.

    Alternative name: Protein TIFY4B

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

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From 17 €