PPH1/TAP38 | Protein phosphatase 1

AS16 4084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana TAP38 sequence, UniProt:P49599, TAIR: AT4G27800

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW 42,7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium hirsutum, Ilex paraguariensis, Mesembryanthemum crystallinum, Nelumbo nucifera, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Theobroma caca, Vigna radiata var. radiata
Species of your interest not listed? Contact us

Application examples

Application example

Western blot using anti/PPH1/TAP38 antibodies

0,5-5 µg of chlorophyll per lane from Arabidopsis thaliana leaves WT (1-4) and delta-TAP38 (5-8), ecotype Columbia were extracted according to Järvi et al 2016 (Plant Physiol 171:1333-1343) and denatured with SDS and 5% B-mercaptoethanol at 65°C for 10 min. After spinning down the proteins were separated on 12% mini-SDS-PAGE with 6 M urea and blotted 1h to PVDF using semi-dry transfer (Hoefer). Membranes were blocked with 5 % milk 0,05% TBS-T for 1h at room temperature (RT) with slow agitation and after a brief rinsing with TBS-T they were incubated in the primary antibody at a dilution of 1: 1000 1% milk TBS-T overnight at 4°C with slow agitation. The antibody solution was decanted and the blot was rinsed briefly twice and washed once for 10 min with fast agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:25 000 in 1 % milk TBS-T for 1h at RT with slow agitation. The blot was washed as above and developed for 5 min with ECL Western Blotting Detection Reagent. Exposure time with Fuji X-ray films for optimal developing was 3 min.

Courtesy of Maija Lespinasse, University of Turku, Finland

Additional information

Background

Background PPH1/TAP38 (Protein phosphatase 1) is a choroplast protein phosphatase TAP38/PPH1, required for efficient dephosphorylation of the LHCII anthena and state transition from state 2 to state 1.

Product citations

Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium hirsutum, Ilex paraguariensis, Mesembryanthemum crystallinum, Nelumbo nucifera, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Theobroma caca, Vigna radiata var. radiata Species of your interest not listed? Contact us
calculated \| apparent molecular mass [kDa]:	42,7 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana TAP38 sequence, UniProt:P49599, TAIR: AT4G27800
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 2000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
background:	PPH1/TAP38 (Protein phosphatase 1) is a choroplast protein phosphatase TAP38/PPH1, required for efficient dephosphorylation of the LHCII anthena and state transition from state 2 to state 1.
Picture (footer):	Application example 0,5-5 µg of chlorophyll per lane from Arabidopsis thaliana leaves WT (1-4) and delta-TAP38 (5-8), ecotype Columbia were extracted according to Järvi et al 2016 (Plant Physiol 171:1333-1343) and denatured with SDS and 5% B-mercaptoethanol at 65°C for 10 min. After spinning down the proteins were separated on 12% mini-SDS-PAGE with 6 M urea and blotted 1h to PVDF using semi-dry transfer (Hoefer). Membranes were blocked with 5 % milk 0,05% TBS-T for 1h at room temperature (RT) with slow agitation and after a brief rinsing with TBS-T they were incubated in the primary antibody at a dilution of 1: 1000 1% milk TBS-T overnight at 4°C with slow agitation. The antibody solution was decanted and the blot was rinsed briefly twice and washed once for 10 min with fast agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:25 000 in 1 % milk TBS-T for 1h at RT with slow agitation. The blot was washed as above and developed for 5 min with ECL Western Blotting Detection Reagent. Exposure time with Fuji X-ray films for optimal developing was 3 min. Courtesy of Maija Lespinasse, University of Turku, Finland

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