PR-2 | Pathogenesis-related protein 2
AS12 2366 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Agostis stolonifera cv. ‘Penncross’, Glycine max

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-2 UniProt P33157, TAIR AT3G57260
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
37.3 kDa (processing aa 1-30, mature peptide 34.1 kD)
Reactivity
Confirmed reactivity
Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Glycine max (roots)
Predicted reactivity
Brassica juncea, Brassica oleracea, Citrus chinensis, Glycine max, Litchi chinensis, Manihot esculenta, Nicotiana tabacum
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example

10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.
Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada

10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.
Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada
Additional information
Additional information
This product can be sold containing Proclin if requested
Does not cross-react with other 1,3-beta glucosidases.
Background
Background
PR-2 (Pathogenesis-related protein 2) is involved in the defence of plants against pathogens. This protein has a catalytic activity and is hydrolysing of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans, EC=3.2.1.39. Alternative names:Glucan endo-1,3-beta-glucosidase, acidic isoform,(1->3)-beta-glucan endohydrolase, Beta-1,3-endoglucanase, Beta-1,3-glucanase 2
Product citations
Selected references
Dong et al. (2020). Overexpression of BrAFP1 gene from winter rapeseed (Brassica rapa) confers cold tolerance in Arabidopsis. Plant Physiol Biochem. 2020 Jul 25;155:338-345.doi: 10.1016/j.plaphy.2020.07.011.
Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ?Penncross?)
Kim et al. (2014). The Arabidopsis Immune Adaptor SRFR1 Interacts with TCP Transcription Factors thatRedundantly Contribute to Effector-Triggered Immunity. Plant J. 2014 Apr 1. doi: 10.1111/tpj.12527.
Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ?Penncross?)
Kim et al. (2014). The Arabidopsis Immune Adaptor SRFR1 Interacts with TCP Transcription Factors thatRedundantly Contribute to Effector-Triggered Immunity. Plant J. 2014 Apr 1. doi: 10.1111/tpj.12527.
Related products: PR-2 | Pathogenesis-related protein 2
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 25 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
Off...
Off...
194 €
AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. ben...
327 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items