PR-4 | Pathogenesis-related protein 4 (Arabidopsis thaliana)

Product no: AS12 2369

AS12 2369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-4 protein sequence, UniProt:P43082 , TAIR:AT3G04720

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 5000 (WB)
    Expected | apparent MW: 22,9 kDa (propeptide), mature peptide 20,7 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Capsicum chinense , Carica papaya, Chimonanthus praecox, Drosera adelae, Eutrema japonicum, Ficus pumila var. awkeotsang , Hevea brasiliensis, Hordeum vulgare, Glycine max, Medicago truncatula, Morus notabilis, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Prunus dulcis, Ricinus communis, Solanum tuberosum, Theobroma cacao, Triticum aestivum, Triticum urartu , Vitis pseudoreticulata
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-PR-4 antibodies

    Arabidopsis thaliana
    leaves treated or not with Pseudomonas syringae containing the avirulence gene avrB: extracted with Tris-HCl 50mM pH 7.8, 0.1 mM EDTA, Triton X-100 0.2% and denatured with SDS 2% and DTT 10mM at 95ºC for 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1: 25 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL Plus (Amersham). Exposure time was 45 seconds.

    Courtesy of Dr. María C. Romero-Puertas, CSIC, Spain
  • Background
  • Background:

    PR-4 (Pathogenesis-related protein 4) involved in defence response. Similar to the antifungal chitin-binding protein hevein from rubber tree latex. mRNA levels increase in response to ethylene and turnip crinkle virus infection.

  • Protocols


  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info
img missing
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 26 €

AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. bentamiana, S. oleracea, S. lycopersicum, T. aestivum, Z. mays, V. vinifera

338 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS12 2366 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Agostis stolonifera cv. ‘Penncross’Glycine max

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
Close