PR-4 | Pathogenesis-related protein 4 (Arabidopsis thaliana)
AS12 2369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-4 protein sequence, UniProt:P43082 , TAIR:AT3G04720
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 ĩl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Expected | apparent MW
22.9 kDa (propeptide), mature peptide 20.7 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Capsicum chinense , Carica papaya, Chimonanthus praecox, Drosera adelae, Eutrema japonicum, Ficus pumila var. awkeotsang , Hevea brasiliensis, Hordeum vulgare, Glycine max, Medicago truncatula, Morus notabilis, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Prunus dulcis, Ricinus communis, Solanum tuberosum, Theobroma cacao, Triticum aestivum, Triticum urartu , Vitis pseudoreticulata
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
Arabidopsis thaliana leaves treated or not with Pseudomonas syringae containing the avirulence gene avrB: extracted with Tris-HCl 50mM pH 7.8, 0.1 mM EDTA, Triton X-100 0.2% and denatured with SDS 2% and DTT 10mM at 95ºC for 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1: 25 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL Plus (Amersham). Exposure time was 45 seconds.
Courtesy of Dr. María C. Romero-Puertas, CSIC, Spain
Arabidopsis thaliana leaves treated or not with Pseudomonas syringae containing the avirulence gene avrB: extracted with Tris-HCl 50mM pH 7.8, 0.1 mM EDTA, Triton X-100 0.2% and denatured with SDS 2% and DTT 10mM at 95ºC for 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1: 25 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL Plus (Amersham). Exposure time was 45 seconds.
Courtesy of Dr. María C. Romero-Puertas, CSIC, Spain
Additional information
Background
Background
PR-4 (Pathogenesis-related protein 4) involved in defence response. Similar to the antifungal chitin-binding protein hevein from rubber tree latex. mRNA levels increase in response to ethylene and turnip crinkle virus infection.
Product citations
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