PR-4 | Pathogenesis-related protein 4 (Arabidopsis thaliana)

AS12 2369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-4 protein sequence, UniProt:P43082 , TAIR:AT3G04720

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000-1 : 5000 (WB)

Expected | apparent MW

22.9 kDa (propeptide), mature peptide 20.7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Capsicum chinense , Carica papaya, Chimonanthus praecox, Drosera adelae, Eutrema japonicum, Ficus pumila var. awkeotsang , Hevea brasiliensis, Hordeum vulgare, Glycine max, Medicago truncatula, Morus notabilis, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Prunus dulcis, Ricinus communis, Solanum tuberosum, Theobroma cacao, Triticum aestivum, Triticum urartu , Vitis pseudoreticulata
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-PR-4 antibodies

Arabidopsis thaliana leaves treated or not with Pseudomonas syringae containing the avirulence gene avrB: extracted with Tris-HCl 50mM pH 7.8, 0.1 mM EDTA, Triton X-100 0.2% and denatured with SDS 2% and DTT 10mM at 95ºC for 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1: 25 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL Plus (Amersham). Exposure time was 45 seconds.

Courtesy of Dr. María C. Romero-Puertas, CSIC, Spain

Additional information

Related products

AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies
AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thaliana
AS07 208 | PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana
AS07 207 | PR-3 | CHN | class I chitinase, rabbit antibodies
AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies
AS13 2724 | PR-10 | Pathogenesis-related protein 10

collection of antibodies to other proteins involved in a response to pathogen attack

Plant protein extraction buffer

Secondary antibodies

Background

PR-4 (Pathogenesis-related protein 4) involved in defence response. Similar to the antifungal chitin-binding protein hevein from rubber tree latex. mRNA levels increase in response to ethylene and turnip crinkle virus infection.