PR-4 | Pathogenesis-related protein 4 (Arabidopsis thaliana)
AS12 2369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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22.9 kDa (propeptide), mature peptide 20.7 kDa
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Arabidopsis thaliana leaves treated or not with Pseudomonas syringae containing the avirulence gene avrB: extracted with Tris-HCl 50mM pH 7.8, 0.1 mM EDTA, Triton X-100 0.2% and denatured with SDS 2% and DTT 10mM at 95ºC for 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1: 25 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL Plus (Amersham). Exposure time was 45 seconds.
Courtesy of Dr. María C. Romero-Puertas, CSIC, Spain
PR-4 (Pathogenesis-related protein 4) involved in defence response. Similar to the antifungal chitin-binding protein hevein from rubber tree latex. mRNA levels increase in response to ethylene and turnip crinkle virus infection.