PsbTn | Tn protein of PSII
AS06 171 | Clonality: | Polyclonal | Host: Rabbit | Arabidopsis thaliana
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For reconstitution add 200 µl of sterile water.
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
no confirmed exceptions from predicted reactivity known in the moment
10 µg of protein from Arabidopsis thaliana thylakoids (Th), BBY, stroma (St), lumen (LU) were separated on 16.5 % SDS-PAGE (Tris-Tricine mini-gel containing 6 M urea) and blotted 1h to PVDF using semi-dry. Blot was blocked with 5 % milk for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 2x for 10 min with TBS and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in a matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent. Exposure time was 3 seconds.
Courtesy of Patrik Storm, Umeå University, Sweden
Add acetone to final concentration of 80% ice-cold acetone.
Leave 10 minutes.
Rresuspend pellet in solubilisation buffer and load on a gel.