showForm Anti-PsbTn antibody

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PsbTn | Tn protein of PSII

AS06 171 | Clonality: | Polyclonal | Host: Rabbit | Arabidopsis thaliana

PsbTn | Tn protein of PSII in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS06 171)

DATA SHEET IN PDF

Qty: 
363
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana PsbTn protein sequence, UniProt:Q39195, TAIR: AT3G21055
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution

For reconstitution add 200 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 11 | 5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Cicer arietinum, Glycine soja, Helianthus annuus, Medicago truncatula, Nicotiana attenuata, Nicotiana tabacum, Nicotiana sylvestris, Noccaea caerulescens, Petunia hybrida, Populus trichocarpa, Solanum chacoense, Solanum tuberosum
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples Application example

Western blot using anti-PsbTn antibodies

10 µg of protein from Arabidopsis thaliana thylakoids (Th), BBY, stroma (St), lumen (LU) were separated on  16.5 % SDS-PAGE (Tris-Tricine mini-gel containing 6 M urea) and blotted 1h to PVDF using semi-dry. Blot was blocked with 5 % milk for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 2x for 10 min with TBS and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in a matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent. Exposure time was 3 seconds. 

Courtesy of Patrik Storm, Umeå University, Sweden

Additional information

LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel.

Protocol: 
Add acetone to final concentration of 80% ice-cold acetone.
Leave 10 minutes. 
Spin.
Rresuspend pellet in solubilisation buffer and load on a gel.

Related products

Background

Background PsbTn (Tn protein of PSII) nuclear encoded protein involved in photosynthesis. Alternative names: photosystem II 5 kDa protein, chloroplastic, PSII-T, Nuclear encoded psbT.

Product citations

Selected references Chen et al. (2019).  The Low Molecular Mass Photosystem II Protein PsbTn is Important for Light Acclimation. Plant Physiol. Apr;179(4):1739-1753. doi: 10.1104/pp.18.01251.

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