Psc | Polycomb group protein Psc
AS17 4129 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
GST-conjugated, full length Psc protein of Drosophila melanogaster, UniProt: P35820
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromating Immunoprecipitation (ChIP)
Expected | apparent MW
169 | 190 kDa
Reactivity
Confirmed reactivity
Drosophila melanogaster
Application examples
Application examples

ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen.

ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen.
Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.
Additional information
Reactivity of this antibody in Western blot requires to be determined
Background
Background
Psc is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
Alternative names: Protein posterior sex combs
Alternative names: Protein posterior sex combs
Product citations
Selected references
To be added when available, antibody released in November 2020.
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