Psc | Polycomb group protein Psc

Name: Psc | Polycomb group protein Psc
SKU: AS17 4129
Price: 296 EUR
Availability: InStock

AS17 4129 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster

Psc | Polycomb group protein Psc in the group Antibodies Other Species / Drosophila melanogaster at Agrisera AB (Antibodies for research) (AS17 4129)

Product Information

Immunogen GST-conjugated, full length Psc protein of Drosophila melanogaster, UniProt: P35820

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromating Immunoprecipitation (ChIP)

Expected | apparent MW

169 | 190 kDa

Reactivity

Confirmed reactivity Drosophila melanogaster

Application examples

Application examples ChIP using anti-Psc antibodies

ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen.

Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.

Additional information

Reactivity of this antibody in Western blot requires to be determined

Background

Background Psc is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

Alternative names: Protein posterior sex combs

Product citations

Selected references To be added when available, antibody released in November 2020.

immunogen:	GST-conjugated, full length Psc protein of Drosophila melanogaster, UniProt: P35820
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromating Immunoprecipitation (ChIP)
calculated \| apparent molecular mass [kDa]:	169 \| 190 kDa
Confirmed reactivity:	Drosophila melanogaster
Picture (footer):	ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen. Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.
additional information (application):	Reactivity of this antibody in Western blot requires to be determined
background:	Psc is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Alternative names: Protein posterior sex combs
All references:	To be added when available, antibody released in November 2020.

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