Psc | Polycomb group protein Psc
AS17 4129 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster
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For reconstitution add 50 µl of sterile water.
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
169 | 190 kDa
ChIP and qPCR analysis were done as described (Schwartz et al., 2006. doi: 10.1038/ng1817). Chromatin from Psc/Su(z)2-KO cells (Kahn et al., 2016. doi: 10.1093/nar/gkw701 ) served as a negative control (Psc minus), chromatin from Ras3 cells served as a positive control (WT). Quantitative PCR was performed with primers specific for BXD-PRE of the Ubx gene (Polycomb target gene in the repressed state) and for an intergenic region (negative control). Figure 1 shows the ChIP recovery, measured by qPCR as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA). Chromatin from 5x107 cells and 3g of anti-Psc antibody were used for each ChIP reaction. For ChIP 3µg of anti-Psc antibody is recommended. Recombinant protein GST-Psc containing 822-1021 aa of Psc / UniProtKB - P35820 (PSC_DROME) / was used as immunogen.
Courtesy of Dr. Tatyana Khan, Umeå University, Sweden.
Alternative names: Protein posterior sex combs