Rabbit anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse and rat serum

AS10 659 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)


product information
Background		Rabbit anti-goat IgG is a secondary antibody conjugated to HRP which binds to goat IgGs in immunological assays.
Immunogen		Purified Goat IgG, whole molecule
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Purified goat IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution		1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Expected \| apparent MW
Confirmed reactivity		Goat IgG heavy chains and light chains on all Goat immunoglobulins
Predicted reactivity		Goat IgG Heavy chains and Light chains on all Goat immunoglobulins
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		No reactivity is observed to non-immunoglobulin goat serum proteins. No reactivity is observed to serum from human, mouse and rat based on immunoelecrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Selected references		to be added when available

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