Rabbit anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse and rat serum
AS10 659 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)
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Product Information
Immunogen
Purified Goat IgG, whole molecule
Host
Rabbit
Clonality
Polyclonal
Purity
Purified goat IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Reactivity
Confirmed reactivity
Goat IgG heavy chains and light chains on all Goat immunoglobulins
Predicted reactivity
Goat IgG Heavy chains and Light chains on all Goat immunoglobulins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin goat serum proteins. No reactivity is observed to serum from human, mouse and rat based on immunoelecrophoresis.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Background
Background
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP which binds to goat IgGs in immunological assays.
Product citations
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