RPL1 | 50S ribosomal protein L1
AS11 1738 | Clonality: Polyclonal | Host: Rabbit | Reactivity:cyanobacteria
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5 µg of total protein from Anabaena sp. (1), Synechococcus sp. 7942 (2), Synechocystis sp. 6803 (3) extracted with Agrisera protein extraction buffer PEB were separated on 4-12% NuPAGE and blotted 1h to PVDF. Blots were blocked with ECL Advance Blocking Reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Healthcare). Exposure time was 180 seconds.
Rpl1 (50S ribosomal protein L1) belongs to the ribosomal protein L1P family. This protein binds directly to 23S rRNA, acts also as a translational repressor protein.