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RPL33 | 50S ribosomal protein L33 (chloroplastic)
AS16 4082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
- Product Info
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Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana RPL33 UniProt: P56796 , TAIR: AtCg00640
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: for reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1: 2000 - 1: 5000 (WB)
Expected | apparent MW: 7,6 | 10 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays
Predicted reactivity: Acacia restiacea, Berberis bealei , Blossfeldia liliputana, Boehmeria spicata, Braya humilis, Calochortus albus, Citrullus colocynthis, Corymbia gummifera, Croomia heterosepala, Croton texensis, Cucurbita pepo, Dodonaea viscosa, Eucalyptus salmonophloia, Fragaria ananassa, Gossypium populifolium, Hippophae rhamnoides, Holodiscus discolor, Khaya senegalensis, Lessertia frutescens, Morus alba var. atropurpurea, Natsiatum herpeticum, Oryza sativa, Pachycladon cheesemanii , Pereskia sacharosa, Polylepis sp., Potentilla lancinata, Ranzania japonica, Ribes fasciculatum var. chinense, Rubus niveus, Solanum lycopersicum, Solanum tuburosum, Sonneratia alba, Trifolium semipilosum, Typha domingensis, Urophysa rockii, Vicia faba , Vicia sepium, Ximenia americana
Species of your interest not listed? Contact usNot reactive in: cyanobacteria - Application Examples
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Application example
10 µg of stromal protein from Arabidopsis thaliana (1) and Zea mays (2) denatured denatured at 95°C 10 minutes were separated on 4-20% SDS-PAGE gradient gel and blotted 1h to PVDF using wet transfer (80V 1 h 30 minutes). Blot was blocked with 5 % milk in TBS-T 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:20 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent and touch exposure was applied.
Courtesy of Dr. Louis-Valentin Mereignier, CNRS, France
- Additional Information
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Additional information (application): Ccross reacting bands may appear when total cell extract is analysed, - Background
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Background: RPL33 | 50S ribosomal protein L33 (chloroplastic) involved in translation process, large subunit of a ribosomal complex. Belongs to bacterial, ribosomal protein bl33 family. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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