RPL33 | 50S ribosomal protein L33 (chloroplastic)
AS16 4082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
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KLH-conjugated peptide, derived from Arabidopsis thaliana RPL33 UniProt: P56796 , TAIR: AtCg00640
1: 2000 - 1: 5000 (WB)
Acacia restiacea, Berberis bealei , Blossfeldia liliputana, Boehmeria spicata, Braya humilis, Calochortus albus, Citrullus colocynthis, Corymbia gummifera, Croomia heterosepala, Croton texensis, Cucurbita pepo, Dodonaea viscosa, Eucalyptus salmonophloia, Fragaria ananassa, Gossypium populifolium, Hippophae rhamnoides, Holodiscus discolor, Khaya senegalensis, Lessertia frutescens, Morus alba var. atropurpurea, Natsiatum herpeticum, Oryza sativa, Pachycladon cheesemanii , Pereskia sacharosa, Polylepis sp., Potentilla lancinata, Ranzania japonica, Ribes fasciculatum var. chinense, Rubus niveus, Solanum lycopersicum, Solanum tuburosum, Sonneratia alba, Trifolium semipilosum, Typha domingensis, Urophysa rockii, Vicia faba , Vicia sepium, Ximenia americana
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10 µg of stromal protein from Arabidopsis thaliana (1) and Zea mays (2) denatured denatured at 95°C 10 minutes were separated on 4-20% SDS-PAGE gradient gel and blotted 1h to PVDF using wet transfer (80V 1 h 30 minutes). Blot was blocked with 5 % milk in TBS-T 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:20 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent and touch exposure was applied.
Courtesy of Dr. Louis-Valentin Mereignier, CNRS, France
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