RPL33 | 50S ribosomal protein L33 (chloroplastic)

AS16 4082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

Product Information

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana RPL33 UniProt: P56796 , TAIR: AtCg00640

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution

for reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution

1: 2000 - 1: 5000 (WB)

Expected | apparent MW

7.6 | 10 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Zea mays

Predicted reactivity

Acacia restiacea, Berberis bealei , Blossfeldia liliputana, Boehmeria spicata, Braya humilis, Calochortus albus, Citrullus colocynthis, Corymbia gummifera, Croomia heterosepala, Croton texensis, Cucurbita pepo, Dodonaea viscosa, Eucalyptus salmonophloia, Fragaria ananassa, Gossypium populifolium, Hippophae rhamnoides, Holodiscus discolor, Khaya senegalensis, Lessertia frutescens, Morus alba var. atropurpurea, Natsiatum herpeticum, Oryza sativa, Pachycladon cheesemanii , Pereskia sacharosa, Polylepis sp., Potentilla lancinata, Ranzania japonica, Ribes fasciculatum var. chinense, Rubus niveus, Solanum lycopersicum, Solanum tuburosum, Sonneratia alba, Trifolium semipilosum, Typha domingensis, Urophysa rockii, Vicia faba , Vicia sepium, Ximenia americana

Species of your interest not listed? Contact us

Not reactive in cyanobacteria

Application examples

Application example

10 µg of stromal protein from Arabidopsis thaliana (1) and Zea mays (2) denatured denatured at 95°C 10 minutes were separated on 4-20% SDS-PAGE gradient gel and blotted 1h to PVDF using wet transfer (80V 1 h 30 minutes). Blot was blocked with 5 % milk in TBS-T 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:20 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent and touch exposure was applied.

Courtesy of Dr. Louis-Valentin Mereignier, CNRS, France

Additional information

Ccross reacting bands may appear when total cell extract is analysed.

Related products

Plant protein extraction buffer

Secondary antibodies

Background

Background RPL33 | 50S ribosomal protein L33 (chloroplastic) involved in translation process, large subunit of a ribosomal complex. Belongs to bacterial, ribosomal protein bl33 family.