IKP

RuvA (protein, positive control)

Product no: AS21 4543P
AS21 4543PProtein/positive control for Western blot
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Delivery:  3-6 business days
  • Product Info
  • Purity: Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 90 % pure by SDS-PAGE.
    Format: Liquid
    Quantity: 20 g
    Storage: Store at 4C or -20C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Expected | apparent MW: 22 kDa (monomer)
  • Application Examples
  • RuvA protein (E.coli), positive control

    10, 5 and 2 µg of highly purified RuvA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.
  • Additional Information
  • Additional information: This product can be used in functional studies as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein
    Additional information (application): RuvA protein is full length, highly purified (over 90 %, SDS-PAGE). UniProt: P0A809
  • Background
  • Background: RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
  • Product Citations
  • Selected references: Han et al. (2006). Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11544-8. doi: 10.1073/pnas.0600753103. Epub 2006 Jul 24. PMID: 16864792; PMCID: PMC1544206.
    Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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