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RuvA (protein, positive control)

AS21 4543PProtein/positive control for Western blot
RuvA (protein, positive control) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4543P)

DATA SHEET IN PDF

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420
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Product name, number (Agrisera, Sweden)

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Product Information

Purity Over 90 %, SDS-PAGE
Format Liquid, 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Quantity 20 µg
Storage Store at 4°C or -20°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Expected | apparent MW 22 kDa (monomer)

Reactivity

Application examples

Application examples RuvA protein (E.coli), positive control

10, 5 and 2 µg of highly purified RuvA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.

Additional information

Additional information This product can be used in functional studies as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein.
RuvA protein is full length, highly purified (over 90 %, SDS-PAGE). UniProt: P0A809

Related products

Related products AS21 4543 | Anti-RuvA | Holliday junction ATP-dependent DNA helicase, rabbit antibodies

Background

Background RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.

Product citations

Selected references Han et al. (2006). Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11544-8. doi: 10.1073/pnas.0600753103. Epub 2006 Jul 24. PMID: 16864792; PMCID: PMC1544206.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.

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