IKP

RuvA (protein, positive control)

Name: RuvA (protein, positive control)
Brand: IKP
SKU: AS21 4543P
Price: 473 EUR
Availability: InStock

Product no: AS21 4543P

AS21 4543P | Protein/positive control for Western blot

473 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Purity:	Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 90 % pure by SDS-PAGE.
Format:	Liquid
Quantity:	20 µg
Storage:	Store at 4°C or -20°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Expected \| apparent MW:	22 kDa (monomer)

Application Examples
10, 5 and 2 µg of highly purified RuvA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.
Additional Information

Additional information:	This product can be used in functional studies as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein
Additional information (application):	RuvA protein is full length, highly purified (over 90 %, SDS-PAGE). UniProt: P0A809

Background

Background:

RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.

Product Citations

Selected references:

Han et al. (2006). Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11544-8. doi: 10.1073/pnas.0600753103. Epub 2006 Jul 24. PMID: 16864792; PMCID: PMC1544206.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.

Protocols
Agrisera Western Blot protocol and video tutorials
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