RPS14 | 40S ribosomal protein S14-1

Product no: AS12 2111

AS12 2111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays, C. reinhardtii, S. lycopersicum

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from a N-terminal of Arabidopsis thaliana  Q9SIH0

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    16 | 15 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays
    Predicted reactivity: Brassica napus, Candidia albicans,, Fusarium oxysporum, Lupinus luteus, Nannochloropsis gaditana, Nicotiana benthamiana, Ostreococcus tauri, Oryza sativa, Picea sitchensis, Populus trichocarpa, Sorghum bicolor, Ricinus communis, Chicken, Human, Mouse, Rat, Salmon, Trypanosoma brucei
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot detection of S14 protein in Arabidopsis

    20 µg of total protein from Arabidopsis thaliana extracts from (1) total cell, nuclei (2), chloroplasts (3), thylakoids (4) were extracted with preparation buffer (330 mM sorbitol, 25mM Tricine pH 7.8, 1mM EDTA, 10mM KCl, 0,15% BSA, 4mM Na ascorbat and 7mM L-cysteine) and separated on 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted 1h 30min to Immobilon™PVDF (Millipore) membrane. Blots were blocked with 10% dry milk in Tris-buffered saline TBS-T (50 mM tris, 150 mM NaCl, pH 7.6 + 1ML 20% TWEEN 20) for 1h at room temperature (RT) with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2 000 (in TBS-T) overnight at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Pierce) diluted to 1:20 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ThermoSuper Signal WestPico according to the manufacturers instructions. Exposure time was 3 minutes. Courtesy of Dr. Rikard Fristedt, UCLA, USA

     

    western blot detection of S14 in various species

     

    5 µg of total protein from Arabidopsis thaliana (1), Zea mays (2), Chlamydomonas reinhardtii (3), Salmo salar (4)  extracted with PEB  were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 2 minutes.

  • Additional Information
  • Additional information (application): 20 µg of total protein is needed for detection of S14 in Arabidopsis thaliana
  • Background
  • Background:

    40S ribosomal protein S14-1 is localized in cytoplasm and belongs to ribosomal protein S11P family.

  • Product Citations
  • Selected references: Pereira Firmino et al. (2020). Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes. Plants (Basel) . 2020 Jul 14;9(7):892.doi: 10.3390/plants9070892.
    Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.
    Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.
    Wegener et al. (2019). Magnetic Tracking of Protein Synthesis in Microfluidic Environments-Challenges and Perspectives. Nanomaterials (Basel). 2019 Apr 9;9(4). pii: E585. doi: 10.3390/nano9040585.
    Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.
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