SAM1-4 | S-adenosylmethionine synthase (affinity purified)

Product no: AS16 3148A

AS16 3148A  |  Clonality: Polyclonal   |  Host: Rabbit   |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:
    KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana SAM1-4, UniProt: P23686, P17562, Q9SJL8, Q9LUT2, TAIR:At1g02500, At4g01850, At3g17390, At2g36880
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 100 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW:

    43.2 | 45 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Malus domestica
    Predicted reactivity:
    Beta vulgaris, Brassica sp., Citrus sp., Coffea canephora, Caomelina sativa, Capsella rubella, Cucumis melo, Cucumis sativus, Genlisea aurea, Gentiana triflora, Guzmania wittmacki, Gossypium raimondii, Eucalyptus grandis, Eutrema salsugineum, Ipomoea batatas, Jatropha curcas, Musa acuminata, Nicotiana sp., Oryza brachyantha, Phoenix dactylifera, Populus sp., Prunus sp., Ricinus communis, Sesamum indicum, Setaria italica, Solanum pennellii, Solanum tuberosum, Spinacia oleracea, Tarenaya hassleriana, Theobroma cacao, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-Sam1-4 antibodies

    70 μg of total protein from Arabidopsis thaliana wt Col-0, SAM3 knockdown, SAM1-4 knockdown, and one oeSAM3-YFP lines extracted with mortar and pestle using 2xSDS loading buffer (100 mM Tris-HCl pH 6.8, 4% SDS, 0.02% bromophenol blue, 200 mM DTT), and denatured in the same buffer at 95°C for 10 min. Samples were separated on 11% SDS-PAGE  and blotted for 1h to PVDF using tank transfer. Blots were blocked with 5% milk powder in TBS-T overnight in a cold room with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed five times for 4 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP-conjugated, from Agrisera) diluted to 1: 20 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent. The proteins were detected using CCD Image Fusion Fx7 after 30 seconds exposure time.


    Courtesy of Louis-Valentin Meteignier, LGBP, Faculté des Sciences de Luminy, France

  • Background
  • Background:
    SAM (S-adenosylmethionin synthetase) is an enzyme which catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps. It is a donor of methyl groups in methylation reactions, and is involved in a number of processes such as: response to cadmium ions and salt stress, as well as, ethylene and lignin biosynthesis.
  • Product Citations
  • Selected references: 38221900
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS16 3148  |  Clonality: Polyclonal   |  Host: Rabbit   |  Reactivity: Arabidopsis thaliana

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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