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SAM1-4 | S-adenosylmethionine synthase (affinity purified)

AS16 3148A  |  Clonality: Polyclonal   |  Host: Rabbit   |  Reactivity: Arabidopsis thaliana

SAM1-4 | S-adenosylmethionine synthase (affinity purified) in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS16 3148A)

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Product Information

Immunogen
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana SAM1-4, UniProt: P23686, P17562, Q9SJL8, Q9LUT2, TAIR:At1g02500, At4g01850, At3g17390, At2g36880
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

43.2 | 45 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Malus domestica
Predicted reactivity
Beta vulgaris, Brassica sp., Citrus sp., Coffea canephora, Caomelina sativa, Capsella rubella, Cucumis melo, Cucumis sativus, Genlisea aurea, Gentiana triflora, Guzmania wittmacki, Gossypium raimondii, Eucalyptus grandis, Eutrema salsugineum, Ipomoea batatas, Jatropha curcas, Musa acuminata, Nicotiana sp., Oryza brachyantha, Phoenix dactylifera, Populus sp., Prunus sp., Ricinus communis, Sesamum indicum, Setaria italica, Solanum pennellii, Solanum tuberosum, Spinacia oleracea, Tarenaya hassleriana, Theobroma cacao, Zea mays

Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-Sam1-4 antibodies

70 μg of total protein from Arabidopsis thaliana wt Col-0, SAM3 knockdown, SAM1-4 knockdown, and one oeSAM3-YFP lines extracted with mortar and pestle using 2xSDS loading buffer (100 mM Tris-HCl pH 6.8, 4% SDS, 0.02% bromophenol blue, 200 mM DTT), and denatured in the same buffer at 95°C for 10 min. Samples were separated on 11% SDS-PAGE  and blotted for 1h to PVDF using tank transfer. Blots were blocked with 5% milk powder in TBS-T overnight in a cold room with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed five times for 4 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP-conjugated, from Agrisera) diluted to 1: 20 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent. The proteins were detected using CCD Image Fusion Fx7 after 30 seconds exposure time.


Courtesy of Louis-Valentin Meteignier, LGBP, Faculté des Sciences de Luminy, France

Additional information

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Background

Background
SAM (S-adenosylmethionin synthetase) is an enzyme which catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps. It is a donor of methyl groups in methylation reactions, and is involved in a number of processes such as: response to cadmium ions and salt stress, as well as, ethylene and lignin biosynthesis.

Product citations

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