showForm Anti-Sar1 (secretion-associated and Ras-related protein 1) antibody, marker of ER

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Sar1 | Secretion-associated and Ras-related protein 1

AS08 326 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, D. sanguinalis, Echinochloa crus-galli E. neomexicana, P. miliaceum, P. maximum, P. vittata, T. aestivum, Z. mays

Sar1 | Secretion-associated and Ras-related protein 1 in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS08 326)

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Product Information

Immunogen

GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7, TAIR: At3g62560

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunogold (IG), Western blot (WB)
Recommended dilution 1 : 50 (IG), 1 : 500 (WB)
Expected | apparent MW

21 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
Predicted reactivity Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Populus trichocarpa, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera
Not reactive in

Nicotiana tabacum

Application examples

Additional information

Additional information

For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.

Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005).  Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17(5):1513-31. Epub 2005 Apr 1.

Related products

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AS08 327 | Anti-Sec21p | gamma subunit, COP vesicles, Golgi marker, rabbit antibodies

AS08 325 | Anti-Arf1 | ADP-ribosylation factor 1 rabbit antibodies

Secondary antibodies

Background

Background

Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Product citations

Selected references Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.
Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 | doi: 10.3389/fgene.2014.00228

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