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Anti-SbtA | Sodium-dependent bicarbonate transporter
AS13 2657 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from known cyanobacterial SbtaA sequences including A0A0A1VVY1, A1XQW8 and others.
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 39 kDa
- Reactivity
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Confirmed reactivity: Cyanobium sp. PCC7001, Synechochoccus elongatus sp.PCC7942, Synechochoccus sp. PCC7002, Synechocystis sp. PCC6803 Predicted reactivity: Cyanobacteria, Cyanothece sp. PCC 7425
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
Negative control, SbtA deletion strain (1), Synechochoccus elongatus PCC7942 (2,3), Cyanobium sp PCC7001 (4), Synechochoccus sp PCC7002 (5), Synechocystis sp PCC6803 (6) extracted with were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed using ATTOPhos.
Courtesy of Dr. Britta Forster, The Australian National University, Canberra, Australia - Additional Information
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Additional information (application): SbtA from Cyanobium PCC7001 is expected to be smaller on SDS-PAGE.
- Background
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Background: SbtA (Sodium-dependent bicarbonate transporter) is an integral membrane protein, involved in Sodium-dependent bicarbonate transport.
- Product Citations
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Selected references: Artier et al. (2019). Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria. Biochim Biophys Acta Bioenerg. 2018 Jun 28. pii: S0005-2728(18)30175-0. doi: 10.1016/j.bbabio.2018.06.015
Artier et al. (2018). Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria. Biochim Biophys Acta. 2018 Jun 28. pii: S0005-2728(18)30175-0. doi: 10.1016/j.bbabio.2018.06.015.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Holland et al. (2016). Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research, 20 (2016) 87–99. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
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