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SbtA | Sodium-dependent bicarbonate transporter

AS13 2657  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: cyanobacteria

SbtA | Sodium-dependent bicarbonate transporter  in the group Antibodies for Plant/Algal  / Photosynthesis  / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS13 2657)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known cyanobacterial SbtaA sequences including A0A0A1VVY1, A1XQW8 and others.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

39 kDa

Reactivity

Confirmed reactivity Cyanobium sp. PCC7001, Synechochoccus elongatus sp.PCC7942, Synechochoccus sp. PCC7002, Synechocystis sp. PCC6803
Predicted reactivity Cyanobacteria
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-SbtA antibodies

Negative control, SbtA deletion strain (1), Synechochoccus elongatus PCC7942 (2,3), Cyanobium sp PCC7001 (4), Synechochoccus sp PCC7002 (5), Synechocystis sp PCC6803  (6) extracted with   were separated on 12  % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed using ATTOPhos.

Courtesy of Dr. Britta Forster, The Australian National University,  Canberra, Australia

Additional information

SbtA from Cyanobium PCC7001 is expected to be smaller on SDS-PAGE.

Related products

Background

Background

SbtA (Sodium-dependent bicarbonate transporter) is an integral membrane protein, involved in Sodium-dependent bicarbonate transport.

Product citations

Selected references Artier et al. (2019). Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria. Biochim Biophys Acta Bioenerg. 2018 Jun 28. pii: S0005-2728(18)30175-0. doi: 10.1016/j.bbabio.2018.06.015
Artier et al. (2018). Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria. Biochim Biophys Acta. 2018 Jun 28. pii: S0005-2728(18)30175-0. doi: 10.1016/j.bbabio.2018.06.015.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Holland et al. (2016). Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research, 20 (2016) 87–99.

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